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Is methylation analysis of SFRP2, TFPI2, NDRG4, and BMP3 promoters suitable for colorectal cancer screening in the Korean population?
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Soo-Kyung Park, Hae Lim Baek, Junghee Yu, Ji Yeon Kim, Hyo-Joon Yang, Yoon Suk Jung, Kyu Yong Choi, Hungdai Kim, Hyung Ook Kim, Kyung Uk Jeong, Ho-Kyung Chun, Kyungeun Kim, Dong Il Park
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Intest Res 2017;15(4):495-501. Published online October 23, 2017
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DOI: https://doi.org/10.5217/ir.2017.15.4.495
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 Abstract
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- <b>Background/Aims</b><br/>
Colorectal cancer (CRC) screening using stool DNA was recently found to yield good detection rates. A multi-target stool DNA test (Cologuard®, Exact Sciences), including methylated genes has been recently approved by the U.S. Food and Drug Administration. The aim of this study was to validate these aberrantly methylated genes as stool-based DNA markers for detecting CRC and colorectal advanced adenoma (AA) in the Korean population. MethodsA single-center study was conducted in 36 patients with AA; 35 patients with CRC; and 40 endoscopically diagnosed healthy controls using CRC screening colonoscopy. The methylation status of the SFRP2, TFPI2, NDRG4, and BMP3 promoters was investigated blindly using bisulfate-modified stool DNA obtained from 111 participants. Methylation status was investigated by methylation-specific polymerase chain reaction. ResultsMethylated SFRP2, TFPI2, NDRG4, and BMP3 promoters were detected in 60.0%, 31.4%, 68.8%, and 40.0% of CRC samples and in 27.8%, 27.8%, 27.8%, and 33.3% of AA samples, respectively. The sensitivities obtained using 4 markers to detect CRC and AA were 94.3% and 72.2%, respectively. The specificity was 55.0%. ConclusionsOur results demonstrate that the SFRP2, TFPI2, NDRG4, and BMP3 promoter methylation analysis of stool sample DNA showed high sensitivity but low specificity for detecting CRC and AA. Because of the low specificity, 4 methylated markers might not be sufficient for CRC screening in the Korean population. Further large-scale studies are required to validate the methylation of these markers in the Asian population and to find new markers for the Asian population.
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Citations
Citations to this article as recorded by  - Effectiveness of single-target fecal DNA methylation test in regional mass screening for colorectal cancer and precancerous lesions in China
Xianhe Kong, Qiuning Wu, Zhi Zhang, Zhiqiang Yu, Feng Niu, Xianshu Wang, Hongzhi Zou
Gastroenterology Report.2025;[Epub] CrossRef - Diagnostic value of genetic and epigenetic biomarker panels for colorectal cancer detection: a systematic review
Georgios Alampritis, Sarah Nohelia Thoukididou, Maria Ramos, Pantelis Georgiou, Melpomeni Kalofonou, Constantinos Simillis
International Journal of Colorectal Disease.2025;[Epub] CrossRef - DNA methylation modulates epigenetic regulation in colorectal cancer diagnosis, prognosis and precision medicine
Jingxin Ye, Jianfeng Zhang, Weifeng Ding
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Chang Kyo Oh, Young-Seok Cho
Intestinal Research.2024; 22(2): 131. CrossRef - Tissue factor pathway inhibitor-2 (TFPI-2)—an underappreciated partaker in cancer and metastasis
Marek Z. Wojtukiewicz, Marta Mysliwiec, Anna Tokajuk, Joanna Kruszewska, Barbara Politynska, Anmbreen Jamroze, Anna M. Wojtukiewicz, Dean G. Tang, Kenneth V. Honn
Cancer and Metastasis Reviews.2024; 43(4): 1185. CrossRef - Detecting colorectal cancer using genetic and epigenetic biomarkers: screening and diagnosis
Yudith Annisa Ayu Rezkitha, Nur Syahadati Retno Panenggak, Maria Inge Lusida, Raissa Virgy Rianda, Isna Mahmudah, Aditya Doni Pradana, Tomohisa Uchida, Muhammad Miftahussurur
Journal of Medicine and Life.2024; 17(1): 4. CrossRef - In Silico Gene Prioritization Highlights the Significance of Bone Morphogenetic Protein 4 (BMP4) Promoter Methylation across All Methylation Clusters in Colorectal Cancer
Daša Jevšinek Skok, Nina Hauptman
International Journal of Molecular Sciences.2023; 24(16): 12692. CrossRef - Novel biomarkers used for early diagnosis and tyrosine kinase inhibitors as targeted therapies in colorectal cancer
Huafeng Jiang, Senjun Zhou, Gang Li
Frontiers in Pharmacology.2023;[Epub] CrossRef - A novel screening method of DNA methylation biomarkers helps to improve the detection of colorectal cancer and precancerous lesions
Yuan Li, Bin Li, Rou Jiang, Leen Liao, Chunting Zheng, Jie Yuan, Liuhong Zeng, Kunling Hu, Yuyu Zhang, Weijian Mei, Zhigang Hong, Binyi Xiao, Lingheng Kong, Kai Han, Jinghua Tang, Wu Jiang, Zhizhong Pan, Shenyan Zhang, Peirong Ding
Cancer Medicine.2023; 12(21): 20626. CrossRef - Current Research on Molecular Biomarkers for Colorectal Cancer in Stool Samples
Patricio Órdenes, Claudio Carril Pardo, Roberto Elizondo-Vega, Karina Oyarce
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Francesco Ferrara, Sofia Zoupanou, Elisabetta Primiceri, Zulfiqur Ali, Maria Serena Chiriacò
Biosensors and Bioelectronics.2022; 196: 113698. CrossRef - Novel Diagnostic Biomarkers in Colorectal Cancer
Aneta L. Zygulska, Piotr Pierzchalski
International Journal of Molecular Sciences.2022; 23(2): 852. CrossRef - Diagnostic accuracy of multitarget stool DNA testing for colorectal cancer screening: A systematic review and meta-analysis
Roya Dolatkhah, Saeed Dastgiri, Mohammad Asghari Jafarabadi, Hossein Mashhadi Abdolahi, Mohammad Hossein Somi
Gastroenterología y Hepatología.2022; 45(10): 753. CrossRef - Technical considerations in PCR-based assay design for diagnostic DNA methylation cancer biomarkers
Maartje Massen, Kim Lommen, Kim A. D. Wouters, Johan Vandersmissen, Wim van Criekinge, James G. Herman, Veerle Melotte, Leo J. Schouten, Manon van Engeland, Kim M. Smits
Clinical Epigenetics.2022;[Epub] CrossRef - DNA methylation biomarkers in colorectal cancer: Clinical applications for precision medicine
Nayeralsadat Fatemi, Sascha Tierling, Hamidreza Aboulkheyr Es, Maryam Varkiani, Ehsan Nazemalhosseini Mojarad, Hamid Asadzadeh Aghdaei, Jörn Walter, Mehdi Totonchi
International Journal of Cancer.2022; 151(12): 2068. CrossRef - Diagnostic accuracy of multitarget stool DNA testing for colorectal cancer screening: A systematic review and meta-analysis
Roya Dolatkhah, Saeed Dastgiri, Mohammad Asghari Jafarabadi, Hossein Mashhadi Abdolahi, Mohammad Hossein Somi
Gastroenterología y Hepatología (English Edition).2022; 45(10): 753. CrossRef - Performance evaluation of stool DNA methylation tests in colorectal cancer screening: a systematic review and meta‐analysis
Mahir Gachabayov, Edward Lebovics, Aram Rojas, Daniel M Felsenreich, Rifat Latifi, Roberto Bergamaschi
Colorectal Disease.2021; 23(5): 1030. CrossRef - Fecal Multidimensional Assay for Non-Invasive Detection of Colorectal Cancer: Fecal Immunochemical Test, Stool DNA Mutation, Methylation, and Intestinal Bacteria Analysis
Shaobo Mo, Hui Wang, Lingyu Han, Wenqiang Xiang, Weixing Dai, Pengfei Zhao, Fengchun Pei, Zhixi Su, Chengcheng Ma, Qi Li, Zhimin Wang, Sanjun Cai, Hao Wang, Rui Liu, Guoxiang Cai
Frontiers in Oncology.2021;[Epub] CrossRef - Identification of DNA methylation markers for early detection of CRC indicates a role for nervous system-related genes in CRC
Glenn Rademakers, Maartje Massen, Alexander Koch, Muriel X. Draht, Nikkie Buekers, Kim A. D. Wouters, Nathalie Vaes, Tim De Meyer, Beatriz Carvalho, Gerrit A. Meijer, James G. Herman, Kim M. Smits, Manon van Engeland, Veerle Melotte
Clinical Epigenetics.2021;[Epub] CrossRef - Advances in Early Detection of Colorectal Cancer: A Focus on Non-invasive Biomarkers
Nadia El Kadmiri
Current Drug Targets.2021; 22(9): 1043. CrossRef - RETRACTED: Using Comorbidity Pattern Analysis to Detect Reliable Methylated Genes in Colorectal Cancer Verified by Stool DNA Test
Yi-Chiao Cheng, Po-Hsien Wu, Yen-Ju Chen, Cing-Han Yang, Jhen-Li Huang, Yu-Ching Chou, Pi-Kai Chang, Chia-Cheng Wen, Shu-Wen Jao, Hsin-Hui Huang, Yi-Hsuan Tsai, Tun-Wen Pai
Genes.2021; 12(10): 1539. CrossRef - Plausibility of an extensive use of stool DNA test for screening advanced colorectal neoplasia
Jiayi Mu, Yanqin Huang, Shanrong Cai, Qilong Li, Yongmao Song, Ying Yuan, Suzhan Zhang, Shu Zheng
Clinica Chimica Acta.2020; 501: 42. CrossRef - Epigenetics of colorectal cancer: biomarker and therapeutic potential
Gerhard Jung, Eva Hernández-Illán, Leticia Moreira, Francesc Balaguer, Ajay Goel
Nature Reviews Gastroenterology & Hepatology.2020; 17(2): 111. CrossRef - Novel biomarkers for the diagnosis and prognosis of colorectal cancer
Hyung-Hoon Oh, Young-Eun Joo
Intestinal Research.2020; 18(2): 168. CrossRef - Fecal DNA methylation markers for detecting stages of colorectal cancer and its precursors: a systematic review
Janhavi R. Raut, Zhong Guan, Petra Schrotz-King, Hermann Brenner
Clinical Epigenetics.2020;[Epub] CrossRef - Hypermethylated Promoters of Secreted Frizzled-Related Protein Genes are Associated with Colorectal Cancer
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Pathology & Oncology Research.2019; 25(2): 567. CrossRef - BMP3 promoter hypermethylation in plasma-derived cell-free DNA in colorectal cancer patients
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Ji Hyun Kim, Sung Chul Park
Gut and Liver.2018; 12(5): 479. CrossRef
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