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Expression of Matrix Metalloproteinase in Colon Adenoma and Colon Cancer: MT1-MMP and TIMP-2
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Jeong Eun Shin, Sung-Ae Jung, Seong-Eun Kim, Yang-Hee Joo*, Ki-Nam Shim, Tae Hun Kim, Kwon Yoo, Il-Hwan Moon
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Intest Res 2007;5(2):144-150. Published online December 30, 2007
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 Abstract
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- Background/Aims
This study investigated the expression of membrane type 1-matrix metalloproteinase (MT1-MMP) and the tissue inhibitor of metalloproteinase-2 (TIMP-2) in cases of adenoma-carcinoma sequence. Methods: Thirty-two samples of colon adenoma, 11 samples of early colon cancer (ECC) and 36 samples of advanced colon cancer (ACC) were collected from colonoscopic biopsies. Normal tissues were also collected from the same subjects. The mRNA expression levels of MT1-MMP and TIMP-2 were quantified using semiquantitative RT-PCR. Results: The mRNA expression levels of MT1-MMP were greater in the ACC samples as compared to the adenoma and ECC samples (p＜0.05, respectively). However, there was no difference in the mRNA expression levels of MT1-MMP between the adenoma and ECC samples. The mRNA expression levels of TIMP-2 were greater in the ACC samples as compared to the adenoma samples (p＜0.001) but did not differ between the adenoma and ECC, and between the ECC and ACC. The expression level of MT1-MMP mRNA was positively related to lymph node metastasis (p＜0.05). However, the mRNA expression levels of MT1-MMP and TIMP-2 did not differ for colon cancer according to differentiation and modified Dukes’ stage. Conclusions: The mRNA expression levels of MT1-MMP and TIMP-2 have limitations as useful markers for malignant degeneration of colonic neoplasm and the progression of colon cancer. (Intest Res 2007;5:144-150)
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