Intestinal Research

Yong Ran Park

1 Article

Colorectal neoplasia

Parthenolide inhibits transforming growth factor β1-induced epithelial-mesenchymal transition in colorectal cancer cells: Shi Mao Zhu, Yong Ran Park, Seung Yong Seo, In Hee Kim, Soo Teik Lee, Sang Wook Kim; Intest Res 2019;17(4):527-536. Published online August 23, 2019; DOI: https://doi.org/10.5217/ir.2019.00031

Background/Aims
Transforming growth factor-β1 (TGF-β1) induction of epithelial-mesenchymal transition (EMT) is one of the mechanisms by which colorectal cancer (CRC) cells acquire migratory and invasive capacities, and subsequently metastasize. Parthenolide (PT) expresses multiple anti-cancer and anti-inflammatory activities that inhibit nuclear factor κB by targeting the IκB kinase complex. In the present study, we aimed to investigate whether PT can inhibit TGF-β1-induced EMT in CRC cell lines.
Methods
HT-29 and SW480 cell lines were used in the experiment. Cell viability was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and sub-G1 analysis was measured by flow cytometry. The induction of EMT by TGF-β1 and inhibition of the process by PT was analyzed by phase contrast microscopy, wounding healing, cellular migration and invasion assays, and Western blotting.
Results
TGF-β1 inhibits HT-29 cell proliferation, but has no effect on SW480 cell proliferation; different concentrations of TGF-β1 did not induce apoptosis in HT-29 and SW480 cells. PT attenuates TGF-β1-induced elongated, fibroblast-like shape changing in cells. PT inhibits TGF-β1-induced cell migration and cell invasion. In addition, other EMT markers such as β-catenin, Vimentin, Snail, and Slug were suppressed by PT, while E-cadherin was increased by PT.
Conclusions
Our findings show that PT inhibits TGF-β1-induced EMT by suppressing the expression of the mesenchymal protein and increasing expression of the epithelial protein. These findings suggest a novel approach for CRC treatment by suppression of TGF-β1-induced EMT.

Citations

Citations to this article as recorded by

Sesquiterpene Lactones as Promising Anti-Glioblastoma Drug Candidates Exerting Complex Effects on Glioblastoma Cell Viability and Proneural–Mesenchymal Transition
Andrey V. Markov, Arseny D. Moralev, Kirill V. Odarenko
Biomedicines.2025; 13(1): 133. CrossRef
Sesquiterpene lactones and cancer: new insight into antitumor and anti-inflammatory effects of parthenolide-derived Dimethylaminomicheliolide and Micheliolide
Jian Li, Xin Li, Hongwei Liu
Frontiers in Pharmacology.2025;[Epub] CrossRef
Sesquiterpene Lactones as Promising Phytochemicals to Cease Metastatic Propagation of Cancer
Fatemeh Mehdikhani, Homa Hajimehdipoor, Mojgan Tansaz, Marc Maresca, Sadegh Rajabi
Biomolecules.2025; 15(2): 268. CrossRef
Cumin (Cuminum cyminum L.) seeds accelerates wound healing in rats: Possible molecular mechanisms
Khaled Abdul‐Aziz Ahmed, Ahmed A. J. Jabbar, Yaseen Galali, Ayman M. Al‐Qaaneh, Gökhan Akçakavak, Nur Ain Salehen, Rawaz Rizgar Hassan, Ramzi A. Mothana, Mahmood Ameen Abdulla, Omer I. Fantoukh, Sidig Hasson, Mohammed F. Hawwal
Skin Research and Technology.2024;[Epub] CrossRef
TGFβ in Pancreas and Colorectal Cancer: Opportunities to Overcome Therapeutic Resistance
Allan M. Johansen, Steven D. Forsythe, Callum T. McGrath, Grayson Barker, Hugo Jimenez, Ravi K. Paluri, Boris C. Pasche
Clinical Cancer Research.2024; 30(17): 3676. CrossRef
NF-κB pathway and angiogenesis: insights into colorectal cancer development and therapeutic targets
Ashkan Bahrami, Amirreza Khalaji, Majed Bahri Najafi, Sina Sadati, Arash Raisi, AmirMohammad Abolhassani, Reza Eshraghi, Mahmood Khaksary Mahabady, Neda Rahimian, Hamed Mirzaei
European Journal of Medical Research.2024;[Epub] CrossRef
Bitter taste signaling in cancer
Ana R. Costa, Ana C. Duarte, Ana R. Costa-Brito, Isabel Gonçalves, Cecília R.A. Santos
Life Sciences.2023; 315: 121363. CrossRef
Parthenolide inhibits proliferation and invasion, promotes apoptosis, and reverts the cell–cell adhesion loss through downregulation of NF‐κB pathway TNF‐α‐activated in colorectal cancer cells
Adriana S. Gehren, Waldemir F. de Souza, Annie C. M. Sousa‐Squiavinato, Diego A. A. Ramos, Bruno R. B. Pires, Eliana S. F. W. Abdelhay, Jose A. Morgado‐Diaz
Cell Biology International.2023; 47(9): 1638. CrossRef
Parthenolide repressed endometriosis induced surgically in rats: Role of PTEN/PI3Kinase/AKT/GSK-3β/β-catenin signaling in inhibition of epithelial mesenchymal transition
Soad L. Kabil, Hayam E. Rashed, Noura Mostafa Mohamed, Nisreen E. Elwany
Life Sciences.2023; 331: 122037. CrossRef
TGF-β in correlation with tumor progression, immunosuppression and targeted therapy in colorectal cancer
Sumeet Singh, Vinita Gouri, Mukesh Samant
Medical Oncology.2023;[Epub] CrossRef
The Emerging Potential of Parthenolide Nanoformulations in Tumor Therapy
Tao An, Huanhuan Yin, Yanting Lu, Feng Liu
Drug Design, Development and Therapy.2022; Volume 16: 1255. CrossRef
Parthenolide reverses the epithelial to mesenchymal transition process in breast cancer by targeting TGFbeta1: In vitro and in silico studies
Hazera Binte Sufian, Julianna Maria Santos, Zeina S. Khan, Sobia Ahsan Halim, Ajmal Khan, Maliha Tabassum Munir, MD Khurshidul Zahid, Ahmed Al-Harrasi, Lauren S. Gollahon, Fazle Hussain, Shaikh Mizanoor Rahman
Life Sciences.2022; 301: 120610. CrossRef
Cinnamomum bejolghota Extract Inhibits Colorectal Cancer Cell Metastasis and TGF-β1-Induced Epithelial-Mesenchymal Transition via Smad and Non-Smad Signaling Pathway
Athicha Kittiwattanokhun, Sukanda Innajak, Etsu Tashiro, Masaya Imoto, Ramida Watanapokasin
Scientia Pharmaceutica.2022; 90(2): 30. CrossRef
Autoimmunity and Cancer—Two Sides of the Same Coin
Justyna Sakowska, Łukasz Arcimowicz, Martyna Jankowiak, Ines Papak, Aleksandra Markiewicz, Katarzyna Dziubek, Małgorzata Kurkowiak, Sachin Kote, Karolina Kaźmierczak-Siedlecka, Karol Połom, Natalia Marek-Trzonkowska, Piotr Trzonkowski
Frontiers in Immunology.2022;[Epub] CrossRef
Sesquiterpene Lactones and Cancer: New Insight into Antitumor and Anti-inflammatory Effects of Parthenolide-Derived Dimethylaminomicheliolide and Micheliolide
Yubo Dong, Xuanjin Qian, Jian Li, Ahmed Faeq Hussein
Computational and Mathematical Methods in Medicine.2022; 2022: 1. CrossRef
Plants as a Source of Anticancer Agents: From Bench to Bedside
Wamidh H. Talib, Safa Daoud, Asma Ismail Mahmod, Reem Ali Hamed, Dima Awajan, Sara Feras Abuarab, Lena Hisham Odeh, Samar Khater, Lina T. Al Kury
Molecules.2022; 27(15): 4818. CrossRef
Pharmacological Targeting of Epithelial-to-Mesenchymal Transition in Colorectal Cancer
Nima Zafari, Mahla Velayati, Mohammadreza Nassiri, Majid Khazaei, Seyed Mahdi Hassanian, Gordon A. Ferns, Amir Avan
Current Pharmaceutical Design.2022; 28(28): 2298. CrossRef
The mechanism of sitagliptin inhibition of colorectal cancer cell lines' metastatic functionalities
Rubén Varela‐Calviño, Marta Rodríguez‐Quiroga, Patrícia Dias Carvalho, Flavia Martins, André Serra‐Roma, Lorena Vázquez‐Iglesias, María Páez de la Cadena, Sérgia Velho, Oscar J. Cordero
IUBMB Life.2021; 73(5): 761. CrossRef
Parthenolide Inhibits Angiogenesis in Esophageal Squamous Cell Carcinoma Through Suppression of VEGF

Bo Tian, Yuhang Xiao, Junliang Ma, Wei Ou, Hui Wang, Jie Wu, Jinming Tang, Baihua Zhang, Xiaojuan Liao, Desong Yang, Zhining Wu, Xu Li, Yong Zhou, Min Su, Wenxiang Wang
OncoTargets and Therapy.2020; Volume 13: 7447. CrossRef
Aniline-containing derivatives of parthenolide: Synthesis and anti-chronic lymphocytic leukaemia activity
Alex S. Quy, Xingjian Li, Louise Male, Tatjana Stankovic, Angelo Agathanggelou, John S. Fossey
Tetrahedron.2020; : 131631. CrossRef
Reversal of Epithelial–Mesenchymal Transition by Natural Anti-Inflammatory and Pro-Resolving Lipids
Chang Hoon Lee
Cancers.2019; 11(12): 1841. CrossRef

9,221 View
188 Download
23 Web of Science
21 Crossref

Author index