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- Infection
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Rapid and accurate diagnosis of
Clostridium difficile infection by real-time polymerase chain reaction - Pil Hun Song, Jung Hwa Min, You Sun Kim, Soo Yeon Jo, Eun Jin Kim, Kyung Jin Lee, Jeonghun Lee, Hyun Sung, Jeong Seop Moon, Dong Hee Whang
- Intest Res 2018;16(1):109-115. Published online January 18, 2018
- DOI: https://doi.org/10.5217/ir.2018.16.1.109
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Abstract
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ePub <b>Background/Aims</b><br/> The incidence and severity of
Clostridium difficile infection (CDI) have increased worldwide, resulting in a need for rapid and accurate diagnostic methods.Methods A retrospective study was conducted to compare CDI diagnosis methods between January 2014 and December 2014. The stool samples, which were obtained in presumptive CDI patients, were compared for their diagnostic accuracy and rapidity, including real-time polymerase chain reaction (PCR) of toxin genes,
C. difficile toxin assay, and culture forC. difficile .Results A total of 207 cases from 116 patients were enrolled in this study and 117 cases (56.5%) were diagnosed as having CDI. Among the 117 cases, the sensitivities of real-time PCR,
C. difficile toxin assay, and culture forC. difficile were 87.2% (102 cases; 95% CI, 80.7%–92.8%), 48.7% (57 cases; 95% CI, 41.0%–59.8%), and 65.0% (76 cases; 95% CI, 60.2%–78.5%), respectively (P <0.005). Notably, 34 cases (29.0%) were diagnosed with CDI by real-time PCR only. The time required to obtain results was 2.27 hours (136.62±82.51 minutes) for real-time PCR, 83.67 hours (5,020.66±3,816.38 minutes) for toxin assay, and 105.79 hours (6,347.68±3,331.46 minutes) for culture (P <0.005), respectively.Conclusions We confirmed that real-time PCR of toxin genes is the most effective diagnostic method for accurate and early diagnosis of CDI. It also helps to diagnose hypervirulent CDI, such as ribotype 027 infection.
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Citations
Citations to this article as recorded by
- Clostridium Difficile Infection in Childhood: Case Series
P. Parusheva, L. Pekova, S. Angelova, B. Bureva
Acta Medica Bulgarica.2025; 52(s1): 98. CrossRef - Usefulness of Stool Multiplex Polymerase Chain Reaction Assays in Patients with Acute Diarrhea
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IEEE Access.2020; 8: 25811. CrossRef - Temporal change of risk factors in hospital-acquired Clostridioides difficile infection using time-trend analysis
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Infection Control & Hospital Epidemiology.2020; 41(9): 1048. CrossRef - Incidence and Characteristics ofClostridioides difficileInfection in Children
Heera Jeong, Ji-Man Kang, Jong Gyun Ahn
Pediatric Infection & Vaccine.2020; 27(3): 158. CrossRef - RecurrentClostridium difficileInfection: Risk Factors, Treatment, and Prevention
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Gut and Liver.2019; 13(1): 16. CrossRef - Is Clostridium difficile infection a real threat in patients with ulcerative colitis? A prospective, multicenter study in Korea
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Intestinal Research.2018; 16(2): 267. CrossRef
- Clostridium Difficile Infection in Childhood: Case Series
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