Skip Navigation
Skip to contents

Intest Res : Intestinal Research

IMPACT FACTOR

Search

Page Path
HOME > Search
2 "Suraj Kumar"
Filter
Filter
Article category
Keywords
Publication year
Authors
Original Articles
Evaluation of Xpert MTB/RIF assay performance in the diagnosis of abdominal tuberculosis
Suraj Kumar, Sawan Bopanna, Saurabh Kedia, Pratap Mouli, Rajan Dhingra, Rajesh Padhan, Mikashmi Kohli, Jigyasa Chaubey, Rohini Sharma, Prasenjit Das, S Dattagupta, Govind Makharia, SK Sharma, Vineet Ahuja
Intest Res 2017;15(2):187-194.   Published online April 27, 2017
DOI: https://doi.org/10.5217/ir.2017.15.2.187
AbstractAbstract PDFPubReaderePub
<b>Background/Aims</b><br/>

The use of genetic probes for the diagnosis of pulmonary tuberculosis (TB) has been well described. However, the role of these assays in the diagnosis of intestinal tuberculosis is unclear. We therefore assessed the diagnostic utility of the Xpert Mycobacterium tuberculosis/rifampicin (MTB/RIF) assay, and estimated the prevalence of multidrug-resistant (MDR) TB in the Indian population.

Methods

Of 99 patients recruited, 37 had intestinal TB; two control groups comprised 43 with Crohn's disease (CD) and 19 with irritable bowel syndrome. Colonoscopy was performed before starting any therapy; mucosal biopsies were subjected to histopathology, acid-fast bacilli staining, Lowenstein-Jensen culture, and nucleic acid amplification testing using the Xpert MTB/RIF assay. Patients were followed up for 6 months to confirm the diagnosis and response to therapy. A composite reference standard was used for diagnosis of TB and assessment of the diagnostic utility of the Xpert MTB/RIF assay.

Results

Of 37 intestinal TB patients, the Xpert MTB/RIF assay was positive in three of 37 (8.1%), but none had MDR-TB. The sensitivity, specificity, positive predictive value, and negative predictive value of the Xpert MTB/RIF assay was 8.1%, 100%, 100%, and, 64.2%, respectively.

Conclusions

The Xpert MTB/RIF assay has low sensitivity but high specificity for intestinal TB, and may be helpful in endemic tuberculosis areas, when clinicians are faced with difficulty differentiating TB and CD. Based on the Xpert MTB/RIF assay, the prevalence of intestinal MDR-TB is low in the Indian population.

Citations

Citations to this article as recorded by  
  • Mistakes to avoid in the management of abdominal tuberculosis
    Abhirup Chatterjee, Daya Krishna Jha, Aravind Sekar, Vishal Sharma
    Expert Review of Anti-infective Therapy.2025; : 1.     CrossRef
  • Non-celiac Enteropathies
    Kaitlyn Mi, Scarlett Cao, Dawn Adams
    Current Gastroenterology Reports.2025;[Epub]     CrossRef
  • Perfusion Computed Tomography May Help in Discriminating Gastrointestinal Tuberculosis and Crohn’s Disease
    Raghav Seth, Pankaj Gupta, Uma Debi, Kaushal Kishore Prasad, Harjeet Singh, Vishal Sharma
    Diagnostics.2023; 13(7): 1255.     CrossRef
  • Clinical characteristics and diagnosis of intestinal tuberculosis in clinical practice at Thailand’s largest national tertiary referral center: An 11-year retrospective review
    Asawin Sudcharoen, Gahwin Ruchikajorndech, Sitthipong Srisajjakul, Ananya Pongpaibul, Popchai Ngamskulrungroj, Orawan Tulyaprawat, Julajak Limsrivilai, Mao-Shui Wang
    PLOS ONE.2023; 18(4): e0282392.     CrossRef
  • Analytical performances of the Xpert MTB/RIF assay using stool specimens to improve the diagnosis of pulmonary tuberculosis in Burkina Faso, a tuberculosis endemic country
    Odilon D. Kaboré, Anselme Millogo, Bintou Sanogo, Emile Birba, Armel Poda, Boubacar Nacro, Olivier Marcy, Sylvain Godreuil, Abdoul-Salam Ouédraogo, Mao-Shui Wang
    PLOS ONE.2023; 18(7): e0288671.     CrossRef
  • Meta-analysis of diagnostic accuracy of nucleic acid amplification tests for abdominal tuberculosis
    Yanqin Shen, Likui Fang, Bo Ye, Guocan Yu, Kamal Kishore Chopra
    PLOS ONE.2023; 18(11): e0289336.     CrossRef
  • Chapitre 7: La tuberculose extrapulmonaire
    Leila Barss, William J. A. Connors, Dina Fisher
    Canadian Journal of Respiratory, Critical Care, and Sleep Medicine.2023; 7(6): 365.     CrossRef
  • Recent updates in diagnosis of abdominal tuberculosis with emphasis on nucleic acid amplification tests
    Preeti Mor, Bhawna Dahiya, Sanjeev Parshad, Pooja Gulati, Promod K. Mehta
    Expert Review of Gastroenterology & Hepatology.2022; 16(1): 33.     CrossRef
  • Chapter 7: Extra-pulmonary tuberculosis
    Leila Barss, William J. A. Connors, Dina Fisher
    Canadian Journal of Respiratory, Critical Care, and Sleep Medicine.2022; 6(sup1): 87.     CrossRef
  • Abdominal Tuberculosis Mimicking Ovarian Cancer: A Case Report and Review of the Literature
    Ikhwan Rinaldi, Abdul Muthalib, Djaja Gosal, Teguh Wijayadi, Barlian Sutedja, Tjondro Setiawan, Andika Gunawan, Nelly Susanto, Lingga Magdalena, Diah Rini Handjari, Fetisari Kurniawan, Aisyah Rifani, Kevin Winston
    International Medical Case Reports Journal.2022; Volume 15: 169.     CrossRef
  • Intestinal Tuberculosis Presenting with Gastrointestinal Bleeding in Patient on Warfarin Therapy
    Werimo Pascal Kuka, Joe Rakiro, Joseph Gatheru, Felix Riunga, Allan Rajula, Daniel C. Damin
    Case Reports in Gastrointestinal Medicine.2022; 2022: 1.     CrossRef
  • Evaluating diagnostic performance of Truenat MTB Plus for gastrointestinal tuberculosis
    Kusum Sharma, Megha Sharma, Vishal Sharma, Megha Sharma, Jayanta Samanta, Aman Sharma, Rakesh Kochhar, Saroj Kant Sinha
    Journal of Gastroenterology and Hepatology.2022; 37(8): 1571.     CrossRef
  • Totally laparoscopic treatment of intestinal tuberculosis complicated with bowel perforation: The first case report in worldwide literature with a brief review
    Giuseppe Di Buono, Roberta Vella, Giuseppe Amato, Giorgio Romano, Vito Rodolico, Marta Saverino, Giovanni De Lisi, Giorgio Romano, Salvatore Buscemi, Antonino Agrusa
    Frontiers in Surgery.2022;[Epub]     CrossRef
  • Acid fast bacillus smear, mycobacterial culture and Xpert MTB/RIF assay for the diagnosis of tuberculous peritonitis in patients with and without end stage renal failure
    Richa Misra, Mitra Kar, Samir Mohindra, Amit Gupta
    Access Microbiology .2022;[Epub]     CrossRef
  • Diagnostic accuracy of the Xpert MTB/RIF assay for abdominal tuberculosis: a systematic review and meta-analysis
    Vishal Sharma, Hariom Soni, Praveen Kumar-M, Saurabh Dawra, Shubhra Mishra, Harshal S Mandavdhare, Harjeet Singh, Usha Dutta
    Expert Review of Anti-infective Therapy.2021; 19(2): 253.     CrossRef
  • Xpert MTB/RIF Ultra and Xpert MTB/RIF assays for extrapulmonary tuberculosis and rifampicin resistance in adults
    Mikashmi Kohli, Ian Schiller, Nandini Dendukuri, Mandy Yao, Keertan Dheda, Claudia M Denkinger, Samuel G Schumacher, Karen R Steingart
    Cochrane Database of Systematic Reviews.2021;[Epub]     CrossRef
  • Intestinal tuberculosis or Crohn's disease: Illusion or delusion or allusion
    Saurabh Kedia, Vineet Ahuja
    JGH Open.2021; 5(2): 177.     CrossRef
  • Application value of tissue tuberculosis antigen combined with Xpert MTB/RIF detection in differential diagnoses of intestinal tuberculosis and Crohn’s disease
    Baoying Fei, Lin Zhou, Yu Zhang, Linhe Luo, Yuanyuan Chen
    BMC Infectious Diseases.2021;[Epub]     CrossRef
  • Gastrointestinal tuberculosis
    Adnan B. Al-Zanbagi, M. K. Shariff
    Saudi Journal of Gastroenterology.2021; 27(5): 261.     CrossRef
  • A combination of circulating microRNA-375-3p and chemokines CCL11, CXCL12, and G-CSF differentiate Crohn’s disease and intestinal tuberculosis
    Susree Roy, Suchandrima Ghosh, Mallica Banerjee, Sayantan Laha, Dipanjan Bhattacharjee, Rajib Sarkar, Sujay Ray, Arko Banerjee, Ranajoy Ghosh, Aniket Halder, Alakendu Ghosh, Raghunath Chatterjee, Simanti Datta, Gopal Krishna Dhali, Soma Banerjee
    Scientific Reports.2021;[Epub]     CrossRef
  • An Outcome-Based Composite Approach for the Diagnosis of Intestinal Tuberculosis: A Pilot Study from a Tertiary Care Centre in South India
    Roopa Rachel Paulose, V Anil Kumar, Aparna Sharma, Aditi Damle, Divya Saikumar, Abish Sudhakar, Anoop K Koshy, Rama P Venu
    Journal of the Royal College of Physicians of Edinburgh.2021; 51(4): 344.     CrossRef
  • Abdominal Tuberculosis in Children: Challenges, Uncertainty, and Confusion
    Giulia Sartoris, James A Seddon, Helena Rabie, Etienne D Nel, H Simon Schaaf
    Journal of the Pediatric Infectious Diseases Society.2020; 9(2): 218.     CrossRef
  • How can gastro-intestinal tuberculosis diagnosis be improved? A prospective cohort study
    Christopher Lowbridge, Soraya A. M. Fadhil, Gayathri D. Krishnan, Emma Schimann, Raman Muthu Karuppan, Nagaraj Sriram, Giri Shan Rajahram, Jayaram Menon, Aatish Patel, Timothy William, Dawn Carmel Paul, Anna P. Ralph
    BMC Infectious Diseases.2020;[Epub]     CrossRef
  • Abdominal Tuberculosis Mimicking Ovarian Cancer: A Diagnostic Dilemma
    Anitha Thomas, Ajit Sebastian, Rachel George, Dhanya Susan Thomas, Grace Rebekah, Priscila Rupali, Joy Sarojini Michael, Abraham Peedicayil
    The Journal of Obstetrics and Gynecology of India.2020; 70(4): 304.     CrossRef
  • Apoptosis in peripheral blood lymphocytes in intestinal tuberculosis and Crohn’s disease: Implications to diagnostic differentiation
    Suprabha Suresh Nayak, Mamatha Vishwanatha Shetty, Cannanore Ganesh Pai, Kanive Parashiva Guruprasad, Kapaettu Satyamoorthy
    Indian Journal of Gastroenterology.2020; 39(4): 338.     CrossRef
  • Tuberculose abdominale
    I. Diallo, T. Omar Soko, A. Rajack Ndiaye, F. Klotz
    EMC - Radiologie et imagerie médicale - Abdominale - Digestive.2020; 38(1): 1.     CrossRef
  • Tuberculose abdominale
    I. Diallo, T. Omar Soko, A. Rajack Ndiaye, F. Klotz
    EMC - Gastro-entérologie.2020; 37(1): 1.     CrossRef
  • Multiplex Polymerase Chain Reaction for diagnosis of gastrointestinal tuberculosis
    Sarthak Malik, Kusum Sharma, Kim Vaiphei, Narendra Dhaka, Neha Berry, Pankaj Gupta, Megha Sharma, Bipadabhanjan Mallick, Rakesh Kochhar, Saroj K Sinha
    JGH Open.2019; 3(1): 32.     CrossRef
  • Differentiating Crohn’s disease from intestinal tuberculosis
    Saurabh Kedia, Prasenjit Das, Kumble Seetharama Madhusudhan, Siddhartha Dattagupta, Raju Sharma, Peush Sahni, Govind Makharia, Vineet Ahuja
    World Journal of Gastroenterology.2019; 25(4): 418.     CrossRef
  • Utility of tissue Xpert-Mtb/Rif for the diagnosis of intestinal tuberculosis in patients with ileocolonic ulcers
    Balaji L Bellam, Harshal S Mandavdhare, Kusum Sharma, Siddharth Shukla, Hariom Soni, Praveen Kumar-M, Harjeet Singh, Kaushal K Prasad, Usha Dutta, Vishal Sharma
    Therapeutic Advances in Infectious Disease.2019;[Epub]     CrossRef
  • GeneXpert in stool: Diagnostic yield in Intestinal Tuberculosis
    Abu Talib, Shaheen Bhatty, Khalid Mehmood, Huda Naim, Iftikhar Haider, Hari Lal, Gohar Ali, Muhammad Nashit
    Journal of Clinical Tuberculosis and Other Mycobacterial Diseases.2019; 17: 100131.     CrossRef
  • GENEXPERT FOR ABDOMINAL TUBERCULOSIS
    Alka SHARMA
    Arquivos de Gastroenterologia.2019; 56(4): 454.     CrossRef
  • A diagnostic approach for differentiating abdominal tuberculosis from ovarian malignancy: a case series and literature review
    Moh Nailul Fahmi, Annisaa Pelita Harti
    BMC Proceedings.2019;[Epub]     CrossRef
  • Xpert®MTB/RIF assay for extrapulmonary tuberculosis and rifampicin resistance
    Mikashmi Kohli, Ian Schiller, Nandini Dendukuri, Keertan Dheda, Claudia M Denkinger, Samuel G Schumacher, Karen R Steingart
    Cochrane Database of Systematic Reviews.2018;[Epub]     CrossRef
  • Surgery for Abdominal Tuberculosis in the Present Era: Experience from a Tertiary-Care Center
    Harjeet Singh, Gautham Krishnamurthy, Jayapal Rajendran, Vishal Sharma, Harshal Mandavdhare, Hemanth Kumar, Thakur Deen Yadav, Rakesh Kumar Vasishta, Rajinder Singh
    Surgical Infections.2018; 19(6): 640.     CrossRef
  • Making a Positive Diagnosis of Intestinal Tuberculosis with the Aid of New Biologic and Histologic Features: How Far Have We Reached?
    Vatsal Mehta, Devendra Desai, Philip Abraham, Camilla Rodrigues
    Inflammatory Intestinal Diseases.2018; 3(4): 155.     CrossRef
  • Editor’s Pick: Recent Advances in the Diagnosis and Management of Abdominal Tuberculosis
    Harshal S. Mandavdhare, Harjeet Singh, Vishal Sharma
    EMJ Gastroenterology.2017; : 52.     CrossRef
  • 7,643 View
  • 100 Download
  • 31 Web of Science
  • 37 Crossref
Close layer
Role of random biopsies in surveillance of dysplasia in ulcerative colitis patients with high risk of colorectal cancer
Sawan Bopanna, Maitreyee Roy, Prasenjit Das, S Dattagupta, V Sreenivas, V Pratap Mouli, Saurabh Kedia, Rajan Dhingra, Rajesh Pradhan, N Suraj Kumar, Dawesh P Yadav, Govind Makharia, Vineet Ahuja
Intest Res 2016;14(3):264-269.   Published online June 27, 2016
DOI: https://doi.org/10.5217/ir.2016.14.3.264
AbstractAbstract PDFPubReaderePub
<b>Background/Aims</b><br/>

Recent data suggest that the incidence of ulcerative colitis (UC) related colorectal cancer (CRC) in India is similar to that of West. The optimum method for surveillance is still a debate. Surveillance with random biopsies has been the standard of care, but is a tedious process. We therefore undertook this study to assess the yield of random biopsy in dysplasia surveillance.

Methods

Between March 2014 and July 2015, patients of UC attending the Inflammatory Bowel Disease clinic at the All India Institute of Medical Sciences with high risk factors for CRC like duration of disease >15 years and pancolitis, family history of CRC, primary sclerosing cholangitis underwent surveillance colonoscopy for dysplasia. Four quadrant random biopsies at 10 cm intervals were taken (33 biopsies). Two pathologists examined specimens for dysplasia, and the yield of dysplasia was calculated.

Results

Twenty-eight patients were included. Twenty-six of these had pancolitis with a duration of disease greater than 15 years, and two patients had associated primary sclerosing cholangis. No patient had a family history of CRC. The mean age at onset of disease was 28.89±8.73 years and the duration of disease was 19.00±8.78 years. Eighteen patients (64.28%) were males. A total of 924 biopsies were taken. None of the biopsies revealed any evidence of dysplasia, and 7/924 (0.7%) were indefinite for dysplasia.

Conclusions

Random biopsy for surveillance in longstanding extensive colitis has a low yield for dysplasia and does not suffice for screening. Newer techniques such as chromoendoscopy-guided biopsies need greater adoption.

Citations

Citations to this article as recorded by  
  • Interlukin-23 inhibitors as an induction and maintenance therapy for moderate to severe ulcerative colitis: a systematic review and meta‑analysis of randomized controlled trials
    Omar Saab, Hasan Al-Obaidi, Marwah Algodi, Asma Algodi, Yasir Rashid, Alhareth Al-Sagban, Hayder Alamily, Nooraldin Merza, Layth Alzubaidy, Andrew DuPont
    Inflammation Research.2025;[Epub]     CrossRef
  • Etrolizumab as an induction and maintenance therapy for ulcerative colitis: A systematic review and meta‐analysis of randomized controlled trials
    Othman Saleh, Mohamed T. Abuelazm, Islam Mohamed, Alaa Ramadan, Mohammad Assaf, Ahmad Alzoubi, Majd M. AlBarakat, Basel Abdelazeem
    JGH Open.2024;[Epub]     CrossRef
  • Low prevalence of primary sclerosing cholangitis in patients with inflammatory bowel disease in India
    Arshdeep Singh, Vandana Midha, Vikram Narang, Saurabh Kedia, Ramit Mahajan, Pavan Dhoble, Bhavjeet Kaur Kahlon, Ashvin Singh Dhaliwal, Ashish Tripathi, Shivam Kalra, Narender Pal Jain, Namita Bansal, Rupa Banerjee, Devendra Desai, Usha Dutta, Vineet Ahuja
    Intestinal Research.2023; 21(4): 452.     CrossRef
  • Colorectal Cancer Surveillance in Patients with Inflammatory Bowel Diseases: Chromoendoscopy or Non-Chromoendoscopy, That Is the Question
    Roberto Gabbiadini, Ferdinando D’Amico, Alessandro De Marco, Maria Terrin, Alessandra Zilli, Federica Furfaro, Mariangela Allocca, Gionata Fiorino, Silvio Danese
    Journal of Clinical Medicine.2022; 11(3): 509.     CrossRef
  • Endoscopic Surveillance in Inflammatory Bowel Diseases: Selecting a Suitable Technology
    Arianna Dal Buono, Roberto Gabbiadini, Federica Furfaro, Marjorie Argollo, Thaís Viana Tavares Trigo, Alessandro Repici, Giulia Roda
    Frontiers in Medicine.2022;[Epub]     CrossRef
  • Heterogeneity of Colorectal Cancer Progression: Molecular Gas and Brakes
    Federica Gaiani, Federica Marchesi, Francesca Negri, Luana Greco, Alberto Malesci, Gian Luigi de’Angelis, Luigi Laghi
    International Journal of Molecular Sciences.2021; 22(10): 5246.     CrossRef
  • Histopathological study of endoscopic biopsies of large intestine
    Manjiri N Karandikar, Purva Kulkarni, Smita Mulay, R C Nimbergi, N S Mani
    Indian Journal of Pathology and Oncology.2021; 8(3): 340.     CrossRef
  • Is histological remission of ulcerative colitis achievable?
    Oleg V. Knyazev, Anna V. Kagramanova, Sergei G. Khomeriki, Asfold I. Parfenov
    Terapevticheskii arkhiv.2021; 93(8): 975.     CrossRef
  • A Systematic Review and Meta-Analysis on the Association between Inflammatory Bowel Disease Family History and Colorectal Cancer
    Hadis Najafimehr, Hamid Asadzadeh Aghdaei, Mohamad Amin Pourhoseingholi, Hamid Mohaghegh Shalmani, Amir Vahedian-Azimi, Matthew Kroh, Mohammad Reza Zali, Amirhossein Sahebkar, Muhammad Naeem
    Gastroenterology Research and Practice.2021; 2021: 1.     CrossRef
  • Safety and effectiveness of adalimumab in the treatment of ulcerative colitis: results from a large-scale, prospective, multicenter, observational study
    Haruhiko Ogata, Takashi Hagiwara, Takeshi Kawaberi, Mariko Kobayashi, Toshifumi Hibi
    Intestinal Research.2021; 19(4): 419.     CrossRef
  • Histological remission of ulcerative colitis with combined anti-cytokine and cell therapy
    Oleg V. Knyazev, Sergey G. Khomeriki, Аnna V. Kagramanova, Albina A. Lishchinskaya, Olga A. Smirnova, Karina K. Noskova, Asfold I. Parfenov
    Terapevticheskii arkhiv.2021; 93(12): 1435.     CrossRef
  • Discovery and Validation of Methylation Biomarkers for Ulcerative Colitis Associated Neoplasia
    Andrew D Beggs, Jonathan James, Germaine Caldwell, Toby Prout, Mark P Dilworth, Phillipe Taniere, Tariq Iqbal, Dion G Morton, Glenn Matthews
    Inflammatory Bowel Diseases.2018; 24(7): 1503.     CrossRef
  • Can histologic remission be a better prognostic factor and therapeutic target beyond endoscopic mucosal healing in patients with ulcerative colitis?
    Hyo Jun Ahn, Sang-Bum Kang
    Intestinal Research.2018; 16(1): 1.     CrossRef
  • The Rising Incidence of Younger Patients With Colorectal Cancer: Questions About Screening, Biology, and Treatment
    Louise C. Connell, José Mauricio Mota, Maria Ignez Braghiroli, Paulo M. Hoff
    Current Treatment Options in Oncology.2017;[Epub]     CrossRef
  • Screening strategy for colorectal cancer according to risk
    Dong Soo Han
    Journal of the Korean Medical Association.2017; 60(11): 893.     CrossRef
  • 6,620 View
  • 59 Download
  • 15 Web of Science
  • 15 Crossref
Close layer

Intest Res : Intestinal Research
Close layer
TOP