Intestinal Research

Original Articles

Inflammatory bowel diseases

Pathophysiological role of Atg5 in human ulcerative colitis: Razieh Ardali, Nasrin Kazemipour, Saeed Nazifi, Kamran Bagheri Lankarani, Iman Razeghian Jahromi, Masood Sepehrimanesh; Intest Res 2020;18(4):421-429. Published online May 8, 2020; DOI: https://doi.org/10.5217/ir.2019.00120

Background/Aims
Ulcerative colitis (UC), along with Crohn’s disease, is one of the main types of inflammatory bowel disease (IBD). On the other hand, deregulated autophagy is involved in many chronic diseases, including IBD. In this study, we aimed to investigate the role of Atg5 and microRNA-181a (miR-181a) in the pathophysiology of UC.
Methods
Colon biopsy, stool, and blood samples of 6 men and 9 women were confirmed for UC. Also, 13 men and 17 women were selected as healthy control (HC). Enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were used to measure the Atg-5 content of the colon biopsies. Besides, the serum and stool levels of Atg5 were measured using ELISA. Moreover, the total RNA of blood cells was extracted and evaluated for the expression of miR-181a.
Results
We found 1.2 ng/mL versus 0.46 ng/mL, 0.34 ng/mL versus 0.24 ng/mL, and 0.082 ng/mL versus 0.062 ng/mL of Atg5 in stool, intestinal tissue, and serum of UC and HCs, respectively. There was no significant difference in the expression of miR-181a in the blood samples of UC and HCs. Immunohistochemistry showed high positivity without any significant difference between the 2 groups in the quantitative analysis.
Conclusions
The significant difference observed between the stool Atg5 content of the HCs and UC patients may provide new insight into using this protein as a diagnostic biomarker, however, considering the small size of our studied population further studies are needed.

Citations

Citations to this article as recorded by

Chronic Gastrointestinal Disorders and miRNA-Associated Disease: An Up-to-Date
Alessandro Giammona, Bruno Giovanni Galuzzi, Elena Imperia, Clarissa Gervasoni, Sofia Remedia, Laura Restaneo, Martina Nespoli, Laura De Gara, Flaminia Tani, Michele Cicala, Michele Pier Luca Guarino, Danilo Porro, Antonio Cerasa, Alessia Lo Dico, Annamar
International Journal of Molecular Sciences.2025; 26(1): 413. CrossRef
Circular RNAs in inflammatory bowel disease: a review of mechanisms, biomarkers and therapeutic potential
Le Yang, Huahui Li, Min Tang, Lingnan He, Lijun Yang
Frontiers in Immunology.2025;[Epub] CrossRef
Pharmacological induction of autophagy reduces inflammation in macrophages by degrading immunoproteasome subunits
Jiao Zhou, Chunxia Li, Meng Lu, Gaoyue Jiang, Shanze Chen, Huihui Li, Kefeng Lu, Hans-Uwe Simon
PLOS Biology.2024; 22(3): e3002537. CrossRef
Autophagy: A potential target for natural products in the treatment of ulcerative colitis
Wei Zhang, Menglong Zou, Jia Fu, Yin Xu, Ying Zhu
Biomedicine & Pharmacotherapy.2024; 176: 116891. CrossRef
Deficiency of Autophagy-Related Gene 5 in Keratinocytes Leads to Aggravation of Epidermal Damage in 2,4-Dinitrochlorobenzene-Induced Allergic Contact Dermatitis
Yi-Qun Zhang, Ta Xiao, Chang-Jun Song, Yang-Ying Ke, Xiang Gao, Min Li, Heng Gu, Xu Chen
International Journal of Dermatology and Venereology.2023; 6(4): 214. CrossRef
Circular RNA HECTD1 Mitigates Ulcerative Colitis by Promoting Enterocyte Autophagy Via miR-182-5p/HuR Axis
Yan Xu, Yuxi Tian, Fujun Li, Ying Wang, Junwen Yang, Hui Gong, Xiaoping Wan, Miao Ouyang
Inflammatory Bowel Diseases.2022; 28(2): 273. CrossRef
Personalized medicine in inflammatory bowel disease: Perspectives on Asia
Su Hyun Park, Sang Hyoung Park
Journal of Gastroenterology and Hepatology.2022; 37(8): 1434. CrossRef
Management of inflammatory bowel disease beyond tumor necrosis factor inhibitors: novel biologics and small-molecule drugs
Soo-Young Na, You Sun Kim
The Korean Journal of Internal Medicine.2022; 37(5): 906. CrossRef
A study on the potential role of autophagy‐related protein 10 as a biomarker for ulcerative colitis
Fatemeh Abbasi Teshnizi, Nasrin Kazemipour, Saeed Nazifi, Kamran Bagheri Lankarani, Masood Sepehrimanesh, Iman Razeghian Jahromi
Physiological Reports.2021;[Epub] CrossRef
Regulation of Endoplasmic Reticulum Stress-Autophagy: A Potential Therapeutic Target for Ulcerative Colitis
Dan Qiao, Ziwei Zhang, Yali Zhang, Qian Chen, Yujun Chen, Yingjue Tang, Xiong Sun, Zhipeng Tang, Yancheng Dai
Frontiers in Pharmacology.2021;[Epub] CrossRef

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IBD

Immunohistochemical differentiation between chronic enteropathy associated with SLCO2A1 gene and other inflammatory bowel diseases: Satoko Yamaguchi, Shunichi Yanai, Shotaro Nakamura, Keisuke Kawasaki, Makoto Eizuka, Noriyuki Uesugi, Tamotsu Sugai, Junji Umeno, Motohiro Esaki, Takayuki Matsumoto; Intest Res 2018;16(3):393-399. Published online July 27, 2018; DOI: https://doi.org/10.5217/ir.2018.16.3.393

Abstract PDF PubReader ePub

<b>Background/Aims</b><br/>

We recently identified recessive mutations in the solute carrier organic anion transporter family member 2A1 gene (SLCO2A1) as causative variants of chronic enteropathy associated with SLCO2A1 (CEAS). The aim of this study was to evaluate SLCO2A1 protein expression in the intestinal tissues of patients with CEAS, intestinal Behçet's disease (BD), simple ulcer (SU), and Crohn's disease (CD).

Methods

Immunohistochemical staining using a polyclonal anti-SLCO2A1 antibody was performed on the resected intestinal specimens from 13 cases of CD, 9 cases of intestinal BD/SU, and 3 cases of CEAS. The extent of SLCO2A1 expression was determined by counting positively-staining vascular endothelial cells and scored as 0 (no cells), 1 (1%–30% cells), 2 (31%–60%), or 3 (>60%). The intensity of SLCO2A1 expression was scored either as 0 (negative), 1 (intermediate), or 2 (strong). The extent score and intensity score were summed for the final score of 0, 2, 3, 4, or 5.

Results

SLCO2A1 protein expression was observed in 1 of 3 cases of CEAS (33%), all 13 cases of CD (100%), and all 9 cases of BD/SU (100%). The mean final expression scores of CEAS, CD, and BD/SU were 1.6 (range, 0–5), 4.8 (range, 4–5), and 4.3 (range, 4–5), respectively. The final expression score in CEAS was significantly lower than in CD (P=0.03).

Conclusions

Immunohistochemical staining of the SLCO2A1 protein is considered useful to distinguish CEAS from other inflammatory bowel diseases.

Citations

Citations to this article as recorded by

Chronic Enteropathy Associated with SLCO2A1 Gene
Junji Umeno, Motohiro Esaki, Keiichi Uchida, Takayuki Matsumoto
Inflammatory Intestinal Diseases.2025; 10(1): 193. CrossRef
First case report of dichorionic diamniotic twins with chronic enteropathy associated with the SLCO2A1 gene
Ryutaro Saura, Shin-ichiro Hagiwara, Keinosuke Hizuka, Nobuhiko Okamoto, Yuri Etani
Clinical Journal of Gastroenterology.2024; 17(2): 240. CrossRef
Characteristics of chronic enteropathy associated with SLCO2A1 gene (CEAS) in children, a unique type of monogenic very early-onset inflammatory bowel disease
Jin Gyu Lim, Jae Sung Ko, Jung Min Ko, Hyun Young Kim, Man Jin Kim, Moon Woo Seong, Young Hun Choi, Gyeong Hoon Kang, Jaemoon Koh, Jin Soo Moon
BMC Pediatrics.2024;[Epub] CrossRef
Clinical and genetic characteristics of Chinese patients diagnosed with chronic enteropathy associated with SLCO2A1 gene
Qing Shang, Yimin Dai, Jingyi Huang, Wei Liu, Weixun Zhou, Yaping Liu, Hong Yang, Qiang Wang, Yue Li
Orphanet Journal of Rare Diseases.2024;[Epub] CrossRef
Histopathology underlying environmental enteric dysfunction in a cohort study of undernourished children in Bangladesh, Pakistan, and Zambia compared with United States children
Paul Kelly, Kelley VanBuskirk, David Coomes, Samer Mouksassi, Gerald Smith, Zehra Jamil, Md Shabab Hossain, Sana Syed, Chelsea Marie, Phillip I Tarr, Peter B Sullivan, William A Petri, Donna M Denno, Tahmeed Ahmed, Mustafa Mahfuz, S Asad Ali, Sean R Moore
The American Journal of Clinical Nutrition.2024; 120: S15. CrossRef
Capsule Endoscopy for the Diagnosis of Suspected Small Bowel Bleeding
P. P. Polyakov, A. Ya. Alimetov, A. V. Onopriev, A. V. Avakimyan, A. Kh. Kade, S. A. Zanin, E. S. Zanina, Z. S. Popov, A. I. Trofimenko, Z. T. Jndoyan, A. A. Avagimyan
Innovative Medicine of Kuban.2023; (3): 121. CrossRef
Attenuated Expression of SLCO2A1 Caused by DNA Methylation in Pediatric Inflammatory Bowel Disease
Natsuki Ito, Takahiro Kudo, Hidetaka Eguchi, Keisuke Jimbo, Atsushi Furuhata, Toshiaki Okuno, Ichiro Takeuchi, Katsuhiro Arai, Takashi Ishige, Yasushi Okazaki, Toshiaki Shimizu
Inflammatory Bowel Diseases.2023; 29(12): 1920. CrossRef
Updates on the diagnosis and management of cryptogenic multifocal ulcerative stenosing enteropathy (CMUSE) and non-steroidal enteropathy
Tom G. Moreels, Ayaskanta Singh
Best Practice & Research Clinical Gastroenterology.2023; 64-65: 101847. CrossRef
Chronic Enteropathy Associated with Solute Carrier Organic Anion Transporter Family, Member 2A1 (SLCO2A1) with Positive Immunohistochemistry for SLCO2A1 Protein
Chizuru Ariake, Naoki Hosoe, Hinako Sakurai, Anna Tojo, Yukie Hayashi, Kenji JL Limpias Kamiya, Tomohisa Sujino, Kaoru Takabayashi, Kenjiro Kosaki, Satowa Seki, Tadakazu Hisamatsu, Haruhiko Ogata, Takanori Kanai
Internal Medicine.2022; 61(17): 2607. CrossRef
Epithelial Abnormalities in the Small Intestine of Zambian Children With Stunting
Chola Mulenga, Sanja Sviben, Kanta Chandwe, Beatrice Amadi, Violet Kayamba, James A. J. Fitzpatrick, Victor Mudenda, Paul Kelly
Frontiers in Medicine.2022;[Epub] CrossRef
Functional analysis of mutant SLCO2A1 transporters found in patients with chronic enteropathy associated with SLCO2A1
Satowa Seki, Gen Tanaka, Toru Kimura, Mari Hayashida, Jun Miyoshi, Minoru Matsuura, Hiroyuki Sakurai, Tadakazu Hisamatsu
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Hee Seung Hong, Jiwon Baek, Jae Chul Park, Ho-Su Lee, Dohoon Park, A-Ran Yoon, Soo Jung Park, Sung Noh Hong, Seong-Joon Koh, Chang Kyun Lee, Bo-In Lee, Sung Wook Hwang, Sang Hyoung Park, Seung-Jae Myung, Suk-Kyun Yang, Kyuyoung Song, Byong Duk Ye
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Small bowel ulcers
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Current Opinion in Gastroenterology.2019; 35(3): 213. CrossRef
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World Journal of Gastroenterology.2019; 25(14): 1753. CrossRef
Distinction between Chronic Enteropathy Associated with the SLCO2A1 Gene and Crohn's Disease
Shunichi Yanai, Satoko Yamaguchi, Shotaro Nakamura, Keisuke Kawasaki, Yosuke Toya, Noriyuki Yamada, Makoto Eizuka, Noriyuki Uesugi, Junji Umeno, Motohiro Esaki, Eiko Okimoto, Shunji Ishihara, Tamotsu Sugai, Takayuki Matsumoto
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Clinics and Research in Hepatology and Gastroenterology.2019; 43(5): e68. CrossRef

9,679 View
122 Download
18 Web of Science
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