Skip Navigation
Skip to contents

Intest Res : Intestinal Research

IMPACT FACTOR

Search

Page Path
HOME > Search
4 "Parthenolide"
Filter
Filter
Article category
Keywords
Publication year
Authors
Original Articles
Colorectal neoplasia
Parthenolide inhibits transforming growth factor β1-induced epithelial-mesenchymal transition in colorectal cancer cells
Shi Mao Zhu, Yong Ran Park, Seung Yong Seo, In Hee Kim, Soo Teik Lee, Sang Wook Kim
Intest Res 2019;17(4):527-536.   Published online August 23, 2019
DOI: https://doi.org/10.5217/ir.2019.00031
AbstractAbstract PDFPubReaderePub
Background/Aims
Transforming growth factor-β1 (TGF-β1) induction of epithelial-mesenchymal transition (EMT) is one of the mechanisms by which colorectal cancer (CRC) cells acquire migratory and invasive capacities, and subsequently metastasize. Parthenolide (PT) expresses multiple anti-cancer and anti-inflammatory activities that inhibit nuclear factor κB by targeting the IκB kinase complex. In the present study, we aimed to investigate whether PT can inhibit TGF-β1-induced EMT in CRC cell lines.
Methods
HT-29 and SW480 cell lines were used in the experiment. Cell viability was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and sub-G1 analysis was measured by flow cytometry. The induction of EMT by TGF-β1 and inhibition of the process by PT was analyzed by phase contrast microscopy, wounding healing, cellular migration and invasion assays, and Western blotting.
Results
TGF-β1 inhibits HT-29 cell proliferation, but has no effect on SW480 cell proliferation; different concentrations of TGF-β1 did not induce apoptosis in HT-29 and SW480 cells. PT attenuates TGF-β1-induced elongated, fibroblast-like shape changing in cells. PT inhibits TGF-β1-induced cell migration and cell invasion. In addition, other EMT markers such as β-catenin, Vimentin, Snail, and Slug were suppressed by PT, while E-cadherin was increased by PT.
Conclusions
Our findings show that PT inhibits TGF-β1-induced EMT by suppressing the expression of the mesenchymal protein and increasing expression of the epithelial protein. These findings suggest a novel approach for CRC treatment by suppression of TGF-β1-induced EMT.

Citations

Citations to this article as recorded by  
  • Sesquiterpene Lactones as Promising Anti-Glioblastoma Drug Candidates Exerting Complex Effects on Glioblastoma Cell Viability and Proneural–Mesenchymal Transition
    Andrey V. Markov, Arseny D. Moralev, Kirill V. Odarenko
    Biomedicines.2025; 13(1): 133.     CrossRef
  • Sesquiterpene lactones and cancer: new insight into antitumor and anti-inflammatory effects of parthenolide-derived Dimethylaminomicheliolide and Micheliolide
    Jian Li, Xin Li, Hongwei Liu
    Frontiers in Pharmacology.2025;[Epub]     CrossRef
  • Sesquiterpene Lactones as Promising Phytochemicals to Cease Metastatic Propagation of Cancer
    Fatemeh Mehdikhani, Homa Hajimehdipoor, Mojgan Tansaz, Marc Maresca, Sadegh Rajabi
    Biomolecules.2025; 15(2): 268.     CrossRef
  • Cumin (Cuminum cyminum L.) seeds accelerates wound healing in rats: Possible molecular mechanisms
    Khaled Abdul‐Aziz Ahmed, Ahmed A. J. Jabbar, Yaseen Galali, Ayman M. Al‐Qaaneh, Gökhan Akçakavak, Nur Ain Salehen, Rawaz Rizgar Hassan, Ramzi A. Mothana, Mahmood Ameen Abdulla, Omer I. Fantoukh, Sidig Hasson, Mohammed F. Hawwal
    Skin Research and Technology.2024;[Epub]     CrossRef
  • TGFβ in Pancreas and Colorectal Cancer: Opportunities to Overcome Therapeutic Resistance
    Allan M. Johansen, Steven D. Forsythe, Callum T. McGrath, Grayson Barker, Hugo Jimenez, Ravi K. Paluri, Boris C. Pasche
    Clinical Cancer Research.2024; 30(17): 3676.     CrossRef
  • NF-κB pathway and angiogenesis: insights into colorectal cancer development and therapeutic targets
    Ashkan Bahrami, Amirreza Khalaji, Majed Bahri Najafi, Sina Sadati, Arash Raisi, AmirMohammad Abolhassani, Reza Eshraghi, Mahmood Khaksary Mahabady, Neda Rahimian, Hamed Mirzaei
    European Journal of Medical Research.2024;[Epub]     CrossRef
  • Bitter taste signaling in cancer
    Ana R. Costa, Ana C. Duarte, Ana R. Costa-Brito, Isabel Gonçalves, Cecília R.A. Santos
    Life Sciences.2023; 315: 121363.     CrossRef
  • Parthenolide inhibits proliferation and invasion, promotes apoptosis, and reverts the cell–cell adhesion loss through downregulation of NF‐κB pathway TNF‐α‐activated in colorectal cancer cells
    Adriana S. Gehren, Waldemir F. de Souza, Annie C. M. Sousa‐Squiavinato, Diego A. A. Ramos, Bruno R. B. Pires, Eliana S. F. W. Abdelhay, Jose A. Morgado‐Diaz
    Cell Biology International.2023; 47(9): 1638.     CrossRef
  • Parthenolide repressed endometriosis induced surgically in rats: Role of PTEN/PI3Kinase/AKT/GSK-3β/β-catenin signaling in inhibition of epithelial mesenchymal transition
    Soad L. Kabil, Hayam E. Rashed, Noura Mostafa Mohamed, Nisreen E. Elwany
    Life Sciences.2023; 331: 122037.     CrossRef
  • TGF-β in correlation with tumor progression, immunosuppression and targeted therapy in colorectal cancer
    Sumeet Singh, Vinita Gouri, Mukesh Samant
    Medical Oncology.2023;[Epub]     CrossRef
  • The Emerging Potential of Parthenolide Nanoformulations in Tumor Therapy
    Tao An, Huanhuan Yin, Yanting Lu, Feng Liu
    Drug Design, Development and Therapy.2022; Volume 16: 1255.     CrossRef
  • Parthenolide reverses the epithelial to mesenchymal transition process in breast cancer by targeting TGFbeta1: In vitro and in silico studies
    Hazera Binte Sufian, Julianna Maria Santos, Zeina S. Khan, Sobia Ahsan Halim, Ajmal Khan, Maliha Tabassum Munir, MD Khurshidul Zahid, Ahmed Al-Harrasi, Lauren S. Gollahon, Fazle Hussain, Shaikh Mizanoor Rahman
    Life Sciences.2022; 301: 120610.     CrossRef
  • Cinnamomum bejolghota Extract Inhibits Colorectal Cancer Cell Metastasis and TGF-β1-Induced Epithelial-Mesenchymal Transition via Smad and Non-Smad Signaling Pathway
    Athicha Kittiwattanokhun, Sukanda Innajak, Etsu Tashiro, Masaya Imoto, Ramida Watanapokasin
    Scientia Pharmaceutica.2022; 90(2): 30.     CrossRef
  • Autoimmunity and Cancer—Two Sides of the Same Coin
    Justyna Sakowska, Łukasz Arcimowicz, Martyna Jankowiak, Ines Papak, Aleksandra Markiewicz, Katarzyna Dziubek, Małgorzata Kurkowiak, Sachin Kote, Karolina Kaźmierczak-Siedlecka, Karol Połom, Natalia Marek-Trzonkowska, Piotr Trzonkowski
    Frontiers in Immunology.2022;[Epub]     CrossRef
  • Sesquiterpene Lactones and Cancer: New Insight into Antitumor and Anti-inflammatory Effects of Parthenolide-Derived Dimethylaminomicheliolide and Micheliolide
    Yubo Dong, Xuanjin Qian, Jian Li, Ahmed Faeq Hussein
    Computational and Mathematical Methods in Medicine.2022; 2022: 1.     CrossRef
  • Plants as a Source of Anticancer Agents: From Bench to Bedside
    Wamidh H. Talib, Safa Daoud, Asma Ismail Mahmod, Reem Ali Hamed, Dima Awajan, Sara Feras Abuarab, Lena Hisham Odeh, Samar Khater, Lina T. Al Kury
    Molecules.2022; 27(15): 4818.     CrossRef
  • Pharmacological Targeting of Epithelial-to-Mesenchymal Transition in Colorectal Cancer
    Nima Zafari, Mahla Velayati, Mohammadreza Nassiri, Majid Khazaei, Seyed Mahdi Hassanian, Gordon A. Ferns, Amir Avan
    Current Pharmaceutical Design.2022; 28(28): 2298.     CrossRef
  • The mechanism of sitagliptin inhibition of colorectal cancer cell lines' metastatic functionalities
    Rubén Varela‐Calviño, Marta Rodríguez‐Quiroga, Patrícia Dias Carvalho, Flavia Martins, André Serra‐Roma, Lorena Vázquez‐Iglesias, María Páez de la Cadena, Sérgia Velho, Oscar J. Cordero
    IUBMB Life.2021; 73(5): 761.     CrossRef
  • Parthenolide Inhibits Angiogenesis in Esophageal Squamous Cell Carcinoma Through Suppression of VEGF


    Bo Tian, Yuhang Xiao, Junliang Ma, Wei Ou, Hui Wang, Jie Wu, Jinming Tang, Baihua Zhang, Xiaojuan Liao, Desong Yang, Zhining Wu, Xu Li, Yong Zhou, Min Su, Wenxiang Wang
    OncoTargets and Therapy.2020; Volume 13: 7447.     CrossRef
  • Aniline-containing derivatives of parthenolide: Synthesis and anti-chronic lymphocytic leukaemia activity
    Alex S. Quy, Xingjian Li, Louise Male, Tatjana Stankovic, Angelo Agathanggelou, John S. Fossey
    Tetrahedron.2020; : 131631.     CrossRef
  • Reversal of Epithelial–Mesenchymal Transition by Natural Anti-Inflammatory and Pro-Resolving Lipids
    Chang Hoon Lee
    Cancers.2019; 11(12): 1841.     CrossRef
  • 9,345 View
  • 188 Download
  • 23 Web of Science
  • 21 Crossref
Close layer
Parthenolide promotes apoptotic cell death and inhibits the migration and invasion of SW620 cells
Yu Chuan Liu, Se Lim Kim, Young Ran Park, Soo-Teik Lee, Sang Wook Kim
Intest Res 2017;15(2):174-181.   Published online April 27, 2017
DOI: https://doi.org/10.5217/ir.2017.15.2.174
AbstractAbstract PDFPubReaderePub
<b>Background/Aims</b><br/>

Parthenolide (PT), a principle component derived from feverfew (Tanacetum parthenium), is a promising anticancer agent and has been shown to promote apoptotic cell death in various cancer cells. In this study, we focused on its functional role in apoptosis, migration, and invasion of human colorectal cancer (CRC) cells.

Methods

SW620 cells were employed as representative human CRC cells. We performed the MTT assay and cell cycle analysis to measure apoptotic cell death. The wound healing, Transwell migration, and Matrigel invasion assays were performed to investigate the effect of PT on cell migration/invasion. Western blotting was used to establish the signaling pathway of apoptosis and cell migration/invasion.

Results

PT exerts antiproliferative effect and induces apoptotic cell death of SW620 cells. In addition, PT prevents cell migration and invasion in a dose-dependent manner. Moreover, PT markedly suppressed migration/invasion-related protein expression, including E-cadherin, β-catenin, vimentin, Snail, cyclooxygenase-2, matrix metalloproteinase-2 (MMP-2), and MMP-9 in SW620 cells. PT also inhibited the expression of antiapoptotic proteins (Bcl-2 and Bcl-xL) and activated apoptosis terminal factor (caspase-3) in a dose-dependent manner.

Conclusions

Our results suggest that PT is a potential novel therapeutic agent for aggressive CRC treatment.

Citations

Citations to this article as recorded by  
  • Sesquiterpene Lactones as Promising Phytochemicals to Cease Metastatic Propagation of Cancer
    Fatemeh Mehdikhani, Homa Hajimehdipoor, Mojgan Tansaz, Marc Maresca, Sadegh Rajabi
    Biomolecules.2025; 15(2): 268.     CrossRef
  • Sesquiterpene lactones and cancer: new insight into antitumor and anti-inflammatory effects of parthenolide-derived Dimethylaminomicheliolide and Micheliolide
    Jian Li, Xin Li, Hongwei Liu
    Frontiers in Pharmacology.2025;[Epub]     CrossRef
  • Cyanidin-3-O-glucoside inhibits the malignant progression of colorectal cancer by regulating Kruppel-like factor 4-mediated ERK/p38 signaling pathway
    Jian Chang, Geqiong Xiao
    Toxicology and Applied Pharmacology.2025; 497: 117268.     CrossRef
  • Novel formulation of parthenolide-loaded liposome coated with chitosan and evaluation of its potential anticancer effects in vitro
    Parisa Karimian Ensaf, Mohammad Taghi Goodarzi, Masoud Homayouni Tabrizi, Ali Neamati, Samira Sadat Hosseinyzadeh
    Molecular Biology Reports.2024;[Epub]     CrossRef
  • Sesquiterpene lactones as emerging biomolecules to cease cancer by targeting apoptosis
    Chou-Yi Hsu, Sadegh Rajabi, Maryam Hamzeloo-Moghadam, Abhinav Kumar, Marc Maresca, Pallavi Ghildiyal
    Frontiers in Pharmacology.2024;[Epub]     CrossRef
  • Exploring the Phytochemistry, Signaling Pathways, and Mechanisms of Action of Tanacetum parthenium (L.) Sch.Bip.: A Comprehensive Literature Review
    Ali Kashkooe, Atefeh Jalali, Mohammad M. Zarshenas, Azadeh Hamedi
    Biomedicines.2024; 12(10): 2297.     CrossRef
  • Bitter taste signaling in cancer
    Ana R. Costa, Ana C. Duarte, Ana R. Costa-Brito, Isabel Gonçalves, Cecília R.A. Santos
    Life Sciences.2023; 315: 121363.     CrossRef
  • Parthenolide inhibits proliferation and invasion, promotes apoptosis, and reverts the cell–cell adhesion loss through downregulation of NF‐κB pathway TNF‐α‐activated in colorectal cancer cells
    Adriana S. Gehren, Waldemir F. de Souza, Annie C. M. Sousa‐Squiavinato, Diego A. A. Ramos, Bruno R. B. Pires, Eliana S. F. W. Abdelhay, Jose A. Morgado‐Diaz
    Cell Biology International.2023; 47(9): 1638.     CrossRef
  • Novel shikonin derivative suppresses tumor growth and metastasis intervention of Wnt/β-catenin pathway
    Hongwei Han, Zhongling Wen, Xiaohui Lai, Minkai Yang, Jiangyan Fu, Liangjie Yang, Qingqing Chen, Yudi Ma, Wencai Jie, Changyi Wang, Tongming Yin, Guihua Lu, Xiaoming Wang, Shucun Sun, Quan Zhao, Jinliang Qi, Hongyan Lin, Yonghua Yang
    Process Biochemistry.2023; 132: 297.     CrossRef
  • Parthenolide repressed endometriosis induced surgically in rats: Role of PTEN/PI3Kinase/AKT/GSK-3β/β-catenin signaling in inhibition of epithelial mesenchymal transition
    Soad L. Kabil, Hayam E. Rashed, Noura Mostafa Mohamed, Nisreen E. Elwany
    Life Sciences.2023; 331: 122037.     CrossRef
  • TGF-β in correlation with tumor progression, immunosuppression and targeted therapy in colorectal cancer
    Sumeet Singh, Vinita Gouri, Mukesh Samant
    Medical Oncology.2023;[Epub]     CrossRef
  • Decoding Systems Biology of Inflammation Signatures in Cancer Pathogenesis: Pan-Cancer Insights from 12 Common Cancers
    Beste Turanli
    OMICS: A Journal of Integrative Biology.2023; 27(10): 483.     CrossRef
  • Parthenolide and Its Soluble Analogues: Multitasking Compounds with Antitumor Properties
    Daniela Carlisi, Marianna Lauricella, Antonella D’Anneo, Anna De Blasio, Adriana Celesia, Giovanni Pratelli, Antonietta Notaro, Giuseppe Calvaruso, Michela Giuliano, Sonia Emanuele
    Biomedicines.2022; 10(2): 514.     CrossRef
  • Parthenolide reverses the epithelial to mesenchymal transition process in breast cancer by targeting TGFbeta1: In vitro and in silico studies
    Hazera Binte Sufian, Julianna Maria Santos, Zeina S. Khan, Sobia Ahsan Halim, Ajmal Khan, Maliha Tabassum Munir, MD Khurshidul Zahid, Ahmed Al-Harrasi, Lauren S. Gollahon, Fazle Hussain, Shaikh Mizanoor Rahman
    Life Sciences.2022; 301: 120610.     CrossRef
  • Sesquiterpene Lactones and Cancer: New Insight into Antitumor and Anti-inflammatory Effects of Parthenolide-Derived Dimethylaminomicheliolide and Micheliolide
    Yubo Dong, Xuanjin Qian, Jian Li, Ahmed Faeq Hussein
    Computational and Mathematical Methods in Medicine.2022; 2022: 1.     CrossRef
  • Recent advances on the structural modification of parthenolide and its derivatives as anticancer agents
    Xingchen LIU, Xiaobing WANG
    Chinese Journal of Natural Medicines.2022; 20(11): 814.     CrossRef
  • Roles of Inflammasomes in Epstein–Barr Virus-Associated Nasopharyngeal Cancer
    Chin King Looi, Ling-Wei Hii, Felicia Fei-Lei Chung, Chun-Wai Mai, Wei-Meng Lim, Chee-Onn Leong
    Cancers.2021; 13(8): 1786.     CrossRef
  • Drug Repurposing to Identify a Synergistic High-Order Drug Combination to Treat Sunitinib-Resistant Renal Cell Carcinoma
    Magdalena Rausch, Adriano Rutz, Pierre-Marie Allard, Céline Delucinge-Vivier, Mylène Docquier, Olivier Dormond, Paul J. Dyson, Jean-Luc Wolfender, Patrycja Nowak-Sliwinska
    Cancers.2021; 13(16): 3978.     CrossRef
  • 6 α-Hydroxy-4[14], 10[15]-guainadien-8β, 12-olide induced cell cycle arrest via modulation of EMT and Wnt/β-catenin pathway in HER-2 positive breast cancer cells
    Gitanjali Javir, Kalpana Joshi, Vijay Khedkar, Supada Rojatkar
    The Journal of Steroid Biochemistry and Molecular Biology.2020; 197: 105514.     CrossRef
  • Parthenolide inhibits ubiquitin-specific peptidase 7 (USP7), Wnt signaling, and colorectal cancer cell growth
    Xue Li, Lingmei Kong, Qihong Yang, Aizhu Duan, Xiaoman Ju, Bicheng Cai, Lin Chen, Tao An, Yan Li
    Journal of Biological Chemistry.2020; 295(11): 3576.     CrossRef
  • Metabonomic study of the intervention effects of Parthenolide on anti-thyroid cancer activity
    Lili Yuan, Zhe Wang, Dongyang Zhang, Jiahe Wang
    Journal of Chromatography B.2020; 1150: 122179.     CrossRef
  • ACT001 can prevent and reverse tamoxifen resistance in human breast cancer cell lines by inhibiting NF‐κB activation
    Xiao‐Han Jin, Yong‐Sheng Jia, Ye‐Hui Shi, Qiu‐Ying Li, Shi‐Qi Bao, Wen‐Ping Lu, Zhong‐Sheng Tong
    Journal of Cellular Biochemistry.2019; 120(2): 1386.     CrossRef
  • Collateral Sensitivity of Parthenolide via NF-κB and HIF-α Inhibition and Epigenetic Changes in Drug-Resistant Cancer Cell Lines
    Mona Dawood, Edna Ooko, Thomas Efferth
    Frontiers in Pharmacology.2019;[Epub]     CrossRef
  • Parthenolide inhibits transforming growth factor β1-induced epithelial-mesenchymal transition in colorectal cancer cells
    Shi Mao Zhu, Yong Ran Park, Seung Yong Seo, In Hee Kim, Soo Teik Lee, Sang Wook Kim
    Intestinal Research.2019; 17(4): 527.     CrossRef
  • Redox Paradox: A Novel Approach to Therapeutics-Resistant Cancer
    Luksana Chaiswing, William H. St. Clair, Daret K. St. Clair
    Antioxidants & Redox Signaling.2018; 29(13): 1237.     CrossRef
  • Parthenolide inhibits tumor-promoting effects of nicotine in lung cancer by inducing P53 - dependent apoptosis and inhibiting VEGF expression
    Wamidh H. Talib, Lina T. Al Kury
    Biomedicine & Pharmacotherapy.2018; 107: 1488.     CrossRef
  • Synergistic effects and mechanisms of impressic acid or acankoreanogein in combination with docetaxel on prostate cancer
    Sen Jiang, Kun Zhang, Yan He, Xuetao Xu, Dongli Li, Shupeng Cheng, Xi Zheng
    RSC Advances.2018; 8(5): 2768.     CrossRef
  • The combined administration of parthenolide and ginsenoside CK in long circulation liposomes with targeted tLyp-1 ligand induce mitochondria-mediated lung cancer apoptosis
    Xin Jin, Jianping Zhou, Zhenhai Zhang, Huixia Lv
    Artificial Cells, Nanomedicine, and Biotechnology.2018; 46(sup3): 931.     CrossRef
  • 7,697 View
  • 97 Download
  • 32 Web of Science
  • 28 Crossref
Close layer
Balsalazide Potentiates Parthenolide-Mediated Inhibition of Nuclear Factor-κB Signaling in HCT116 Human Colorectal Cancer Cells
Hyun-Young Kim, Se-Lim Kim, Young-Ran Park, Yu-Chuan Liu, Seung Young Seo, Seong Hun Kim, In Hee Kim, Seung Ok Lee, Soo Teik Lee, Sang Wook Kim
Intest Res 2015;13(3):233-241.   Published online June 9, 2015
DOI: https://doi.org/10.5217/ir.2015.13.3.233
AbstractAbstract PDFPubReader
<b>Background/Aims</b><br/>

Balsalazide is an anti-inflammatory drug used in the treatment of inflammatory bowel disease. Balsalazide can reduce inflammatory responses via several mechanisms, including inhibition of nuclear factor-κB (NF-κB) activity. Parthenolide (PT) inhibits NF-κB and exerts promising anticancer effects by promoting apoptosis. The present investigated the antitumor effects of balsalazide, combined with PT, on NF-κB in a representative human colorectal carcinoma cell line, HCT116.

Methods

We counted cells and conducted annexin-V assays and cell cycle analysis to measure apoptotic cell death. Western blotting was used investigate the levels of proteins involved in apoptosis.

Results

PT and balsalazide produced synergistic anti-proliferative effects and induced apoptotic cell death. The combination of balsalazide and PT markedly suppressed nuclear translocation of the NF-κB p65 subunit and the phosphorylation of inhibitor of NF-κB. Moreover, PT and balsalazide dramatically enhanced NF-κB p65 phosphorylation. Apoptosis, through the mitochondrial pathway, was confirmed by detecting effects on Bcl-2 family members, cytochrome c release, and activation of caspase-3 and -8.

Conclusions

Combination treatment with PT and balsalazide may offer an effective strategy for the induction of apoptosis in HCT116 cells.

Citations

Citations to this article as recorded by  
  • Roles of Inflammasomes in Epstein–Barr Virus-Associated Nasopharyngeal Cancer
    Chin King Looi, Ling-Wei Hii, Felicia Fei-Lei Chung, Chun-Wai Mai, Wei-Meng Lim, Chee-Onn Leong
    Cancers.2021; 13(8): 1786.     CrossRef
  • Computational investigation of FDA approved drugs as selective PARP-1 inhibitors by targeting BRCT domain for cancer therapy
    Chandan Kumar, P.T.V. Lakshmi, Annamalai Arunachalam
    Journal of Molecular Graphics and Modelling.2021; 108: 107919.     CrossRef
  • Identification of Autophagy-Associated Biomarkers and Corresponding Regulatory Factors in the Progression of Colorectal Cancer
    Chunrui Zhang, Jing Jiang, Liqiang Wang, Liyu Zheng, Jiankai Xu, Xiaolin Qi, Huiying Huang, Jianping Lu, Kongning Li, Hong Wang
    Frontiers in Genetics.2020;[Epub]     CrossRef
  • Anticancer and apoptotic activities of parthenolide in combination with epirubicin in mda-mb-468 breast cancer cells
    Arash Ghorbani-Abdi-Saedabad, Mohammad Yahya Hanafi-Bojd, Negin Parsamanesh, Zahra Tayarani-Najaran, Homa Mollaei, Reyhane Hoshyar
    Molecular Biology Reports.2020; 47(8): 5807.     CrossRef
  • Parthenolide as Cooperating Agent for Anti-Cancer Treatment of Various Malignancies
    Malgorzata Sztiller-Sikorska, Malgorzata Czyz
    Pharmaceuticals.2020; 13(8): 194.     CrossRef
  • Integration of genetic variants and gene network for drug repurposing in colorectal cancer
    Lalu Muhammad Irham, Henry Sung-Ching Wong, Wan-Hsuan Chou, Wirawan Adikusuma, Eko Mugiyanto, Wan-Chen Huang, Wei-Chiao Chang
    Pharmacological Research.2020; 161: 105203.     CrossRef
  • Synthesis of azo dyes possessing N-heterocycles and evaluation of their anticancer and antibacterial properties
    Zarrin Ghasemi, Sajjad Azizi, Roya Salehi, Hossein Samadi Kafil
    Monatshefte für Chemie - Chemical Monthly.2018; 149(1): 149.     CrossRef
  • Parthenolide attenuated bleomycin-induced pulmonary fibrosis via the NF-κB/Snail signaling pathway
    Xiao-he Li, Ting Xiao, Jia-huan Yang, Yuan Qin, Jing-jing Gao, Hui-juan Liu, Hong-gang Zhou
    Respiratory Research.2018;[Epub]     CrossRef
  • Ursodeoxycholic acid inhibits the proliferation of colon cancer cells by regulating oxidative stress and cancer stem-like cell growth
    Eun-Kyung Kim, Jae Hee Cho, EuiJoo Kim, Yoon Jae Kim, Aamir Ahmad
    PLOS ONE.2017; 12(7): e0181183.     CrossRef
  • Dietary selenium protects adiponectin knockout mice against chronic inflammation induced colon cancer
    Arpit Saxena, Raja Fayad, Kamaljeet Kaur, Samantha Truman, Julian Greer, James A. Carson, Anindya Chanda
    Cancer Biology & Therapy.2017; 18(4): 257.     CrossRef
  • Sesquiterpene binding Gly-Leu-Ser/Lys-“co-adaptation pocket” to inhibit lung cancer cell epithelial-mesenchymal transition
    Xiao-Yu Ai, Heng Zhang, Shao-Yan Gao, Yuan Qin, Wei-Long Zhong, Ju Gu, Meng Li, Kai-Liang Qiao, Qin Tian, Zhan-Hong Cui, Jia-Huan Yang, Zhun Bi, Ting Xiao, Shuang Chen, Hui-Juan Liu, Hong-Gang Zhou, Tao Sun, Cheng Yang
    Oncotarget.2017; 8(41): 70192.     CrossRef
  • Juglanin inhibits lung cancer by regulation of apoptosis, ROS and autophagy induction
    Liang Chen, Ya-Qiong Xiong, Jing Xu, Ji-Peng Wang, Zi-Li Meng, Yong-Qing Hong
    Oncotarget.2017; 8(55): 93878.     CrossRef
  • Cyr61 participates in the pathogenesis of acute lymphoblastic leukemia by enhancing cellular survival via the AKT/NF-κB signaling pathway
    Xianjin Zhu, Yanfang Song, Conglian Wu, Chuxi Pan, Pingxia Lu, Meihua Wang, Peizheng Zheng, Rongfen Huo, Chenqing Zhang, Wanting Li, Yulin Lin, Yingping Cao, Ningli Li
    Scientific Reports.2016;[Epub]     CrossRef
  • 6,183 View
  • 44 Download
  • 14 Web of Science
  • 13 Crossref
Close layer
Parthenolide Sensitizes Human Colorectal Cancer Cells to Tumor Necrosis Factor-related Apoptosis-inducing Ligand through Mitochondrial and Caspase Dependent Pathway
Kieu Thi Thu Trang, Se-Lim Kim, Sang-Bae Park, Seung-Young Seo, Chung-Hwan Choi, Jin-Kyoung Park, Jin-Chang Moon, Soo-Teik Lee, Sang-Wook Kim
Intest Res 2014;12(1):34-41.   Published online January 28, 2014
DOI: https://doi.org/10.5217/ir.2014.12.1.34
AbstractAbstract PDFPubReader
<b>Background/Aims</b><br/>

Combination therapy utilizing tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) in conjunction with other anticancer agents, is a promising strategy to overcome TRAIL resistance in malignant cells. Recently, parthenolide (PT) has proved to be a promising anticancer agent, and several studies have explored its use in combination therapy. Here, we investigated the molecular mechanisms by which PT sensitizes colorectal cancer (CRC) cells to TRAIL-induced apoptosis.

Methods

HT-29 cells (TRAIL-resistant) were treated with PT and/or TRAIL for 24 hours. The inhibitory effect on proliferation was detected using the 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Annexin V staining, cell cycle analysis, and Hoechst 33258 staining were used to assess apoptotic cell death. Activation of an apoptotic pathway was confirmed by Western blot.

Results

Treatment with TRAIL alone inhibited the proliferation of HCT 116 cells in a dose-dependent manner, whereas proliferation was not affected in HT-29 cells. Combination PT and TRAIL treatment significantly inhibited cell growth and induced apoptosis of HT-29 cells. We observed that the synergistic effect was associated with misregulation of B-cell lymphoma 2 (Bcl-2) family members, release of cytochrome C to the cytosol, activation of caspases, and increased levels of p53.

Conclusion

Combination therapy using PT and TRAIL might offer an effetive strategy to overcome TRAIL resistance in certain CRC cells.

Citations

Citations to this article as recorded by  
  • Nanomagnetic Liposome-Encapsulated Parthenolide and Indocyanine Green for Targeting and Chemo-Photothermal Antitumor Therapy
    Wenbin Gao, Lei Li, Xuwu Zhang, Liyao Luo, Yuchu He, Cong Cong, Dawei Gao
    Nanomedicine.2020; 15(9): 871.     CrossRef
  • Nano magnetic liposomes-encapsulated parthenolide and glucose oxidase for ultra-efficient synergistic antitumor therapy
    Wenbin Gao, Shipan Wei, Zhuo Li, Lei Li, Xuwu Zhang, Chunhui Li, Dawei Gao
    Nanotechnology.2020; 31(35): 355104.     CrossRef
  • Anticancer and apoptotic activities of parthenolide in combination with epirubicin in mda-mb-468 breast cancer cells
    Arash Ghorbani-Abdi-Saedabad, Mohammad Yahya Hanafi-Bojd, Negin Parsamanesh, Zahra Tayarani-Najaran, Homa Mollaei, Reyhane Hoshyar
    Molecular Biology Reports.2020; 47(8): 5807.     CrossRef
  • Parthenolide as Cooperating Agent for Anti-Cancer Treatment of Various Malignancies
    Malgorzata Sztiller-Sikorska, Malgorzata Czyz
    Pharmaceuticals.2020; 13(8): 194.     CrossRef
  • Down‐regulation of intracellular anti‐apoptotic proteins, particularly c‐FLIP by therapeutic agents; the novel view to overcome resistance to TRAIL
    Ali Hassanzadeh, Majid Farshdousti Hagh, Mohammad Reza Alivand, Ali Akbar Movassaghpour Akbari, Karim Shams Asenjan, Raedeh Saraei, Saeed Solali
    Journal of Cellular Physiology.2018; 233(10): 6470.     CrossRef
  • Parthenolide and DMAPT induce cell death in primitive CML cells through reactive oxygen species
    Gabriela Flores‐Lopez, Dafne Moreno‐Lorenzana, Manuel Ayala‐Sanchez, Socrates Aviles‐Vazquez, Hector Torres‐Martinez, Peter A. Crooks, Monica L. Guzman, Hector Mayani, Antonieta Chávez‐González
    Journal of Cellular and Molecular Medicine.2018; 22(10): 4899.     CrossRef
  • Combined Parthenolide and Balsalazide Have Enhanced Antitumor Efficacy Through Blockade of NF-κB Activation
    Se-Lim Kim, Seong Hun Kim, Young Ran Park, Yu-Chuan Liu, Eun-Mi Kim, Hwan-Jeong Jeong, Yo Na Kim, Seung Young Seo, In Hee Kim, Seung Ok Lee, Soo Teik Lee, Sang-Wook Kim
    Molecular Cancer Research.2017; 15(2): 141.     CrossRef
  • Protective effects of Alpha-lipoic acid on MeHg-induced oxidative damage and intracellular Ca2+dyshomeostasis in primary cultured neurons
    Tianyao Yang, Zhaofa Xu, Wei Liu, Bin Xu, Yu Deng
    Free Radical Research.2016; 50(5): 542.     CrossRef
  • Sesquiterpene lactones of Moquiniastrum polymorphum subsp. floccosum have antineoplastic effects in Walker-256 tumor-bearing rats
    Gracianny Gomes Martins, Francislaine Aparecida dos Reis Lívero, Aline Maria Stolf, Caroline Machado Kopruszinski, Cibele Campos Cardoso, Olair Carlos Beltrame, José Ederaldo Queiroz-Telles, Regiane Lauriano Batista Strapasson, Maria Élida Alves Stefanell
    Chemico-Biological Interactions.2015; 228: 46.     CrossRef
  • Balsalazide Potentiates Parthenolide-Mediated Inhibition of Nuclear Factor-κB Signaling in HCT116 Human Colorectal Cancer Cells
    Hyun-Young Kim, Se-Lim Kim, Young-Ran Park, Yu-Chuan Liu, Seung Young Seo, Seong Hun Kim, In Hee Kim, Seung Ok Lee, Soo Teik Lee, Sang Wook Kim
    Intestinal Research.2015; 13(3): 233.     CrossRef
  • Goniothalamin enhances TRAIL-induced apoptosis in colorectal cancer cells through DR5 upregulation and cFLIP downregulation
    THANET SOPHONNITHIPRASERT, SIRINUN NILWARANGKOON, YUKIO NAKAMURA, RAMIDA WATANAPOKASIN
    International Journal of Oncology.2015; 47(6): 2188.     CrossRef
  • 8,841 View
  • 47 Download
  • 15 Web of Science
  • 11 Crossref
Close layer

Intest Res : Intestinal Research
Close layer
TOP