1Department of Infectious, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.
2Department of Endoscopy, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.
© Copyright 2018. Korean Association for the Study of Intestinal Diseases.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
FINANCIAL SUPPORT: The authors received no financial support for the research, authorship, and/or publication of this article.
CONFLICT OF INTEREST: No potential conflict of interest relevant to this article was reported.
AUTHOR CONTRIBUTION: S.N. and A.H. were involved in conception and design of the study. S.N., A.H., A.I., T.O., Tetsu Kinjo, T.H., Takeshi Kinjo, and G.L.P. were involved in acquisition of data. S.N. and A.H. were involved in drafting of the manuscript. J.F. was involved in supervising the project. All authors were involved in interpretation of data and critical revision of the manuscript and have approved the final manuscript for submission.
Gene | Primer sequence [ref] | Thermal profile | Amplicon size (bp) |
---|---|---|---|
gB | gB1043: 5'-tctgggaagcctcgcaaacg-3' [4] | 35 Cycles: | 680–685 |
gB1724: 5'-gagtagcagcgtcctggcga-3' [4] | 94℃ 5 min, 94℃ 45 s | ||
62℃ 1 min, 72℃ 45 s | |||
72℃ 5 min | |||
gH | gH1 (F): 5'-gagacttaacacctacgcat-3' [17] | 45 Cycles: | 181 |
gH1 (R): 5'-cgatcccttccagtcg-3' [17] | 94℃ 15 min, 94℃ 30 s | ||
55℃ 30 sec, 72℃ 30 s | |||
72℃ 5 min | |||
gH2 (F): 5'-tggacacgatctactattca-3' [17] | 45 Cycles: | 134 | |
gH2 (R): 5'-tgtcgtcgtctatggac-3' [17] | 95℃ 15 min, 95℃ 30 s | ||
55℃ 30 s, 72℃ 30 s | |||
72℃ 5 min | |||
gN | gN (F): 5'-ggcggtggtgtgatggagtg-3' [13] | 35 Cycles: | 418 |
gN (R): 5'-aatagcctttggtggttgc-3' [13] | 96℃ 2 min, 96℃ 30 s | ||
60℃ 30 s, 72℃ 40 s | |||
72℃ 5 min |
Gene | Primer sequence [ref] | Thermal profile | Amplicon size (bp) |
---|---|---|---|
gB | gB1043: 5'-tctgggaagcctcgcaaacg-3' [ | 35 Cycles: | 680–685 |
gB1724: 5'-gagtagcagcgtcctggcga-3' [ | 94℃ 5 min, 94℃ 45 s | ||
62℃ 1 min, 72℃ 45 s | |||
72℃ 5 min | |||
gH | gH1 (F): 5'-gagacttaacacctacgcat-3' [ | 45 Cycles: | 181 |
gH1 (R): 5'-cgatcccttccagtcg-3' [ | 94℃ 15 min, 94℃ 30 s | ||
55℃ 30 sec, 72℃ 30 s | |||
72℃ 5 min | |||
gH2 (F): 5'-tggacacgatctactattca-3' [ | 45 Cycles: | 134 | |
gH2 (R): 5'-tgtcgtcgtctatggac-3' [ | 95℃ 15 min, 95℃ 30 s | ||
55℃ 30 s, 72℃ 30 s | |||
72℃ 5 min | |||
gN | gN (F): 5'-ggcggtggtgtgatggagtg-3' [ | 35 Cycles: | 418 |
gN (R): 5'-aatagcctttggtggttgc-3' [ | 96℃ 2 min, 96℃ 30 s | ||
60℃ 30 s, 72℃ 40 s | |||
72℃ 5 min |
gB, glycoprotein B; gH, glycoprotein H; gN, glycoprotein N; F, forward; R, reverse.
Patient | Sex | Age (yr) | Disease duration (yr) | Extent of disease | Mayo score | Endoscopic Mayo score | Bioptic evidence of CMV | Ongoing medication | Outcome | Detected genotypes of CMV |
---|---|---|---|---|---|---|---|---|---|---|
JPN-1 | M | 60 | 1 | Pancolitis | 9 | 2 | Positive | PSL 2.5 mg | GCV | gB1, gH2 |
Thiopurines | Surgery | |||||||||
Anti-TNF | ||||||||||
5-ASA | ||||||||||
JPN-3 | M | 62 | 15 | Pancolitis | 8 | 2 | Positive | PSL 20 mg | GCV | gB3, gN3b, gH2 |
5-ASA | Improved | |||||||||
JPN-5 | F | 43 | 2 | Proctitis | 8 | 2 | Negative | PSL enema | Improved | gH1 |
5-ASA | ||||||||||
JPN-6 | M | 25 | 2 | Pancolitis | 6 | 1 | ND | PSL enema | Improved | gH2 |
5-ASA | ||||||||||
JPN-7 | M | 53 | 26 | Left-sided colitis | 9 | 2 | ND | PSL enema | Improved | gB1, gN3a, gH1, gH2 |
Thiopurines | ||||||||||
5-ASA | ||||||||||
JPN-8 | M | 58 | 5 | Pancolitis | 9 | 2 | Negative | PSL 40 mg | Improved | gN3a, gH2 |
5-ASA | ||||||||||
JPN-9 | M | 49 | 2 | Pancolitis | 5 | 1 | Negative | PSL 30 mg | Improved | gN3b, gH2 |
Thiopurines | ||||||||||
5-ASA | ||||||||||
JPN-13 | F | 43 | 4 | Left-sided colitis | 9 | 2 | Positive | PSL enema | Improved | gH2 |
5-ASA |
gB, glycoprotein B; gN, glycoprotein N; gH, glycoprotein H; CMV, cytomegalovirus; JPN, Japan; M, male; PSL, prednisolone; GCV, ganciclovir; TNF, tumor necrosis factor; ASA, aminosalicylic acid; F, female; ND, not determined.
gB, glycoprotein B; gH, glycoprotein H; gN, glycoprotein N; F, forward; R, reverse.
gB, glycoprotein B; gN, glycoprotein N; gH, glycoprotein H; CMV, cytomegalovirus; JPN, Japan; M, male; PSL, prednisolone; GCV, ganciclovir; TNF, tumor necrosis factor; ASA, aminosalicylic acid; F, female; ND, not determined.