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Intest Res > Volume 11(4); 2013 > Article
Intestinal Research 2013;11(4):268-275.
DOI:    Published online October 30, 2013.
Near-infrared Fluorescence Imaging Using a Protease-activatable Nanoprobe in Tumor Detection: Comparison with Narrow-band Imaging
Soon Man Yoon, In-Wha Kim, Miyeoun Song, Eun-Ju Do, Ju Hee Ryu2, Kwangmeyung Kim2, Ick Chan Kwon2, Mi Jung Kim3, Dae Hyuk Moon4, Dong-Hoon Yang5, Kyoung Jo Kim5, Byong Duk Ye5, Jeong-Sik Byeon5, Suk-Kyun Yang5, Jin-Ho Kim5, Seung-Jae Myung5
1Department of Internal Medicine, Chungbuk National University College of Medicine, Cheongju, Asan Institute for Life Sciences
2Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology
4Departments of Pathology3 and Nuclear Medicine
5Asan Medical Center, University of Ulsan College of Medicine, Department of Gastroenterology, Asan Digestive Disease Research Institute, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea
Advances in endoscopic technology seek to improve the accuracy of neoplastic tumor detection. Recently developed endoscopy devices such as narrow-band imaging (NBI) nevertheless have limitations in morphologic diagnosis. The purpose of this study was to investigate whether a novel imaging technique—near-infrared fluorescence (NIRF) imaging using a protease-activatable nanoprobe—could provide more accurate neoplastic tumor detection, compared to NBI. Methods: Images of the intestines of ApcMin/+ mice were obtained by NIRF using a matrix metalloproteinase (MMP)-sensing probe, which was based on a nanoparticle platform. Immediately after imaging, endoscopy with NBI capability was performed on the same excised intestine. Macroscopic and microscopic findings in the intestines were assessed, and MMP expression was analyzed by Western blotting and real-time polymerase chain reaction. Results: Numerous tiny polypoid lesions were present in the intestines of aged ApcMin/+ mice. These lesions included adenomas, lymphoid follicles, and protruding normal tissues. When using NIRF imaging with an MMP-activatable nanoprobe, adenomatous polyps showed higher fluorescence, compared to lymphoid follicles or adjacent normal tissues. The expression of MMP was higher in the adenomatous tissue than in the other tissues. The sensitivity and specificity for adenoma detection were 88.9% and 82.2%, respectively, when using NIRF imaging with a MMP-nanoprobe, compared to 77.8% and 66.7%, respectively, when using NBI (P<0.05). Conclusions: Near-infrared fluorescence imaging with a protease-activatable nanoprobe could aid in the differentiation of tumor characteristics. Clinical application of this approach may improve the endoscopic detection of neoplastic tumors. (Intest Res 2013;11:268-275)
Key Words: Molecular imaging, Narrow band imaging, Neoplasms, Diagnosis
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