Does Metformin Affect The Incidence of Colonic Polyps and Adenomas in Patients with Type 2 Diabetes Mellitus?
Article information
Abstract
Background/Aims
Colorectal cancer (CRC) develops from colonic adenomas. Type 2 diabetes mellitus (DM) is associated with a higher risk of CRC and metformin decreases CRC risk. However, it is not certain if metformin affects the development of colorectal polyps and adenomas. This study aimed to elucidate if metforminaffects the incidence of colonic polyps and adenomas in patients with type 2 DM.
Methods
Of 12,186 patients with type 2 DM, 3,775 underwent colonoscopy between May 2001 and March 2013. This study enrolled 3,105 of these patients, and divided them in two groups: 912 patients with metformin use and 2,193 patients without metformin use. Patient clinical characteristics, polyp and adenoma detection rate in the two groups were analyzed retrospectively.
Results
The Colorectal polyp detection rate was lower in the metformin group than in the non-meformin group (39.4% vs. 62.4%, P<0.01). Colorectal adenoma detection rate was significantly lower in the metformin group than in the non-metformin group (15.2% vs. 20.5%, P<0.01). Fewer advanced adenomas were detected in the metformin group than in the non-metformin group (12.2% vs. 22%, P<0.01). Multivariate analysis identified age, sex, Body mass index and metformin use as factors associated with polyp incidence, whereas only metforminwas independently associated with decreased adenoma incidence (Odd ratio=0.738, 95% CI=0.554-0.983, P=0.03).
Conclusions
In patients with type 2 DM, metformin reduced the incidence of adenomas that may transform into CRC. Therefore, metformin may be useful for the prevention of CRC in patients with type 2 DM.
INTRODUCTION
Colorectal cancer (CRC) is the third leading cause of cancer-related deaths worldwide,1 and its prevalence and mortality rates continue to increase in Korea.2 CRC mostly develops from colonic adenomas.3 To reduce the incidence of CRC, the focus has shifted from removal of early-detected adenomas to prevention of the formation of adenomas causing CRC; recent studies have investigated the effects of dietary fiber, calcium, nonsteroidal anti-inflammatory drugs, aspirin, statins, and other medications in the prevention of CRC.4,5 Since CRC is a cancer associated with lifestyle-related diseases such as diabetes mellitus (DM) or obesity, the therapeutic agents used for these diseases have been investigated for the prevention of CRC.6,7 Type 2 DM is a known risk factor for CRC worldwide;8 hyperinsulinemia caused by insulin resistance influences the incidence of CRC.9,10,11 Metformin is a biguanide derivative that inhibits gluconeogenesis and glycogen decomposition, and increases glucose absorption in muscle tissues. Unlike other oral hypoglycemic agents, metformin does not cause hypoglycemia; therefore, it is appropriate for the initial treatment of diabetes.12 Metformin also activates liver kinase B1-dependent AMP-activated protein kinase (AMPK) in the liver, and activated AMPK inhibits the proliferation and growth of cells by inhibiting mammalian target of rapamycin.13 A recent study suggests that AMPK activated by low-dose metformin inhibits the formation of aberrant crypt foci (ACF), a surrogate marker of CRC.14,15 Moreover, it has also been recently reported that the risk of cancers, including CRC, is lower in type 2 DM patients being treated with metformin than those not treated with metformin.9,16,17,18,19,20 Some animal studies have revealed that metformin prevents the proliferation of colonic epithelial cells.21,22 However, it remains to be proven whether the intake of metformin inhibits the occurrence of adenomas, which are the precursor lesions of CRC. In this study, we aimed to investigate if administrating metformin prevented the formation of colonic polyps, particularly adenomas, in patients with type 2 DM.
METHODS
1. Subjects
This study retrospectively reviewed the records of 3,775 patients aged ≥40 years with no family history of CRC and who underwent colonoscopy for the first time from a total cohort of 12,186 patients diagnosed with type 2 DM from May 2001 to March 2013 in Soonchunhyang University Bucheon Hospital. We excluded 124 patients with malignant neoplasm, IBD, or cancers other than CRC, and 546 patients who were administered metformin for <1 month prior to colonoscopy. Adenoma was confirmed based on the pathological findings of endoscopic biopsy. This study enrolled 3,105 patients (Fig. 1), and was performed after approval from the institutional review board of Soonchunhyang University Bucheon Hospital (SCHBC_IRB_2013-64).
2. Methods
We recorded the age, sex, BMI, any associated illnesses, duration of DM, smoking status, use of aspirin and statins, duration of metformin use, and blood test results (triglyceride [TG], total cholesterol, HDL cholesterol, LDL cholesterol, and glycated hemoglobin [HbA1c] levels) during colonoscopy. Since the use of low-dose metformin for >1 month could inhibit the formation of ACF, patients were divided into those with a history of metformin use for >1 month prior to colonoscopy (the metformin group) and those with no history of metformin use (non-metformin group). Polyps detected during colonoscopy were divided into hyperplastic polyps, adenomas, inflammatory polyps, and adenocarcinomas according to histologic findings. Adenomas were classified into tubular, tubulovillous, and villous adenomas according to histologic types, and low grade, moderate grade, and high-grade dysplasias according to differentiation. Adenomas >1 cm, tubulovillous or villous adenomas, high-grade dysplasia, or more than three small-sized adenomas were defined as advanced adenomas.23,24
3. Statistical Analysis
All statistical analyses were performed using the statistical software package SPSS version 14.0 (SPSS Inc., Chicago, IL, USA). All data were presented as mean±SD, numerical values, and percentages. The analysis of continuous variables was conducted using Student t-test, whereas the analysis of categorical variables was performed using the chi-square test. Univariate analysis was performed to compare the polyp detection rate (PDR) and adenoma detection rate (ADR) between the metformin and non-metformin groups. This study investigated if metformin affects the PDRs and ADRs using multivariate logistic regression analysis to modify variables with statistical difference such as age, sex, BMI, and TG levels, and variables that might confound analysis results including duration of DM, use of aspirin and statins, and smoking history. P-values>0.05 were considered statistically significant.
RESULTS
1. Clinical Characteristic of Patients
A total of 3,105 patients were enrolled in the study, with 912 patients in the metformin group and 2,193 patients in the non-metformin group. Mean duration of metformin medication use was 14.7±18.2 months. Patients with metformin use for <1 year accounted for 65.7%, and 12.8% of all subjects used metformin for >3 years. The mean age of patients was significantly lower in the metformin group than in the non-metformin group (60.1±10.8 vs. 63.9±12.0 years, P<0.01). BMI was recorded to determine obesity. Mean BMI was significantly higher in metformin group than in the non-metformin group; it was in the metformin group (25.0±3.7 vs. 24.3±3.6 kg/m2, P<0.01). The metformin group had a larger proportion of patients with BMI >25 kg/m2 than the non-metformin group (48% vs. 40.2%, P<0.01). The proportion of smokers were 11% and 10.5% in the metformin and non-metformin groups, respectively, with no statistical difference between the two groups (P=0.72). The time since diagnosis of DM was longer in the metformin group than in the non-metformin group (11.9±7.8 vs. 9.9±7.1 years, P<0.01). Both total cholesterol and LDL cholesterol levels were higher in the non-metformin group than in the metformin group. No statistical difference was found in HbA1c between the two groups (7.5±3.8 vs. 7.5±4.5 mmol/mol, P=0.65). The proportion of patients using aspirin and statins were significantly higher in the metformin group than in the non-metformin group (aspirin: 53.4%, statins: 64.4%, P<0.01; Table 1).
2. Comparison of the Detection Rate and Characteristics of Polyps
Polyp detection rates (PDRs) were 39.4% and 62.4% in metformin and non-metformin groups, respectively, and were significantly higher in the non-metformin group (P<0.01). The mean number of polyps detected was significantly higher in the non-metformin group than in the metformin group (1.6±2.2 vs. 1.1±1.8, P<0.01). ADRs were 15.2% and 20.5% in the metformin and non-metformin groups, respectively, showing a statistically significantly higher detection rate in the non-metformin group (P<0.01). On the other hand, polyp size did not significantly differ between the groups (42.0±5.6 vs. 37.0±3.8 mm, P=0.14; Table 2).
3. Comparison of the Detection Rate of Polyps and Adenomas in the Metformin Group According to the Duration of Metformin Use
The detection rates of polyps and adenomas were compared in the metformin group according to the duration of metformin use. The PDR was 36.6% in patients taking metformin for <1 year, 47.5% in patients taking metformin for 1-2 years, 42.3% in patients taking metformin for 2-3 years, and 43.6% in patients taking metformin for >3 years, with no statistically significant difference among groups (P=0.07). The ADR was 15.9% in patients taking metformin for <1 year, 13.6% in patients taking metformin for 1-2 years, 14.1% in patients taking metformin for 2-3 years, and 14.5% in patients taking metformin for >3 years, with no statistically significant difference among groups (P=0.59; Fig. 2).
4. Comparison of the Histologic Characteristics of Adenomas
Histologic classification and grade were compared between adenomas detected in the metformin and non-metformin groups. No difference was found in the ratios of tubular, tubulovillous, and villous adenomas between the two groups (P=0.72), or in the proportion of low grade, moderate grade, and high-grade dysplasias (P=0.20; Table 3).
5. Comparison of Advanced Adenomas
Among the study subjects, 6.5% and 7.3% had adenomas >1 cm in the metformin and non-metformin groups, respectively, with no statistically significant difference between the two groups (P=0.73). No statistical difference was found in the detection rate of villous adenomas between the two groups (P=0.98). The detection rate of well-differentiated adenomas was lower in metformin group than in the non-metformin group, with no significant difference between the two groups (2.9% vs. 4.5%, P=0.47). The detection rate of more than three adenomas with a higher recurrence rate was markedly lower in metformin group than in the non-metformin group (12.2% vs. 22%, P<0.01; Table 4).
6. Factors Affecting the Detection Rate of Polyps and Adenomas
Regression analysis was performed to identify whether the use of metformin is the factor that affects the detection rate of polyps and adenomas by modifying variables including age, sex, BMI, TG, HbA1c, duration of DM, smoking status, and use of aspirin and statins. According to the results, age, sex, BMI, and smoking status were identified as factors influencing the PDR, in addition to metformin use (Table 5). Regression analysis conducted by adjusting the above variables to identify factors affecting the ADR showed that metformin use alone reduced the detection rate of adenomas (OR=0.738, 95% CI=0.554-0.983, P=0.03; Table 6).
DISCUSSION
A number of studies have shown that DM is associated with an increased incidence of CRC, breast cancer, endometrial cancer, renal cancer, liver cancer, and other cancers;25 meta-analyses have proven that the relative risk of CRC is higher in patients with DM.26 Insulin-like growth factor-1 (IGF-1) level increases due to hyperinsulinemia resulting from insulin resistance in DM patients, and elevated IGF-1 levels affect the incidence of colonic adenomas and CRC inducing proliferation and dysplasia of normal and carcinoma cells.27 The incidence and mortality rates of CRC are showing a continuously increasing trend worldwide.2 Along with the removal of early-detected CRC or colonic adenomas, the use of preventive medicines is anticipated to be effective in preventing CRC. The most common cause of CRC is development from colonic adenomas after a series of genetic variations. Although the association of DM with colonic adenomas has been verified,28,29 and metformin has been proven to lower the risk of CRC in patients with type 2 DM,30 results of different studies that have investigated whether metformin decreases the incidence of colonic polyps or adenomas are inconsistent. A Korean study showed that metformin reduced the incidence of colonic adenomas in patients with a history of CRC.31 For these reasons, this study compared the detection rate of colonic polyps and adenomas in 3,105 patients with type 2 DM aged ≥40 years with no history of CRC and who were undergoing colonoscopy for the first time by dividing them into two groups: a metformin and non-metformin group. The detection rates of both polyps and adenomas were found to be significantly lower in the metformin group than in the non-metformin group, implying that metformin prevents the incidence of colonic polyps and the occurrence of colonic adenomas, which are precursor lesions of CRC. Although no difference was found in the histologic classification and grade of colonic adenomas between the two groups, the incidence of three colonic adenomas was significantly lower in the metformin group than in the non-metformin group. According to the multivariate analysis performed to identify factors that affect the detection rate of colonic adenomas, age, sex, BMI, TG, HbA1c, smoking status, and DM duration were not found to be associated with the incidence of colonic adenomas. Unlike previous studies that suggested that the use of aspirin and statins was related with the occurrence of colonic adenomas,32,33 the risk of colonic adenomas was not reduced in patients treated with aspirin or statins in this study. In contrast, metformin use decreased the risk of colonic adenoma incidence by 37%. Therefore, metformin may be effective in preventing the development of colonic adenomas in patients with type 2 DM. This study has a few limitations. It was a single-center retrospective study. Since the study mainly relied on medical records without evaluating the clinical characteristics of patients or the use of aspirin or statins, the duration of medication use was indeterminable. Therefore, the outcome of this study cannot be compared with that of previous studies. Furthermore, the skill-level of physicians who performed colonoscopy for polyp detection was not standardized, and colon cleanliness and colonoscopy withdrawal time were not evaluated as variables that may have affected the detection rate. Unlike previous studies, this study identified the effectiveness of metformin in lowering the occurrence of colonic adenomas in patients with type 2 DM by comparing the detection rates of polyps and adenomas in a large number of subjects with type 2 DM stratified according to metformin use. Large-scale, prospective, randomized studies with various controlled confounding variables will be needed to overcome the study limitations and clearly prove the efficacy of metformin in preventing colonic adenomas.
Notes
Financial support: None.
Conflict of interest: None.