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- Original Article BioflorⰒ Suppresses Expression of Interleukin-8 in HT-29 cell
- Sang Kil Lee, Hyo Jong Kim, Sung Gil Chi, Jae Young Jang, Ki Deok Nam, Nam Hoon Kim, Kwang Ro Joo, Seok Ho Dong, Byung Ho Kim, Young Woon Chang, Joung Il Lee, Rin Chang
- Intestinal Research 2004;2(2):96-101. Published online: December 22, 2004
Department of Internal Medicine, Kyung Hee University College of Medicine, Department of Internal Medicine and Pathology, Kyung Hee University College of Medicine, Seoul, Korea
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Abstract
Background/Aims
Nowadays, there is a growing interest in probiotics as a safe way of changing the intestinal bacterial flora. Probiotics may have potential in several gastroenterological conditions. The nonpathogenic yeast, Saccharomyces boulardii (S. boulardii, BioflorⰒ), has been used empirically in the treatment of acute infectious diarrhea and antibiotics-associated diarrhea. Recently, S. boulardii was reported to be beneficial in the treatment of inflammatory bowel disease, however, little is known about their mechanisms of action. We examined the ability of S. boulardii to modulate inflammatory response in human colon cells. Methods: Effects of BioflorⰒ on survival and proliferation of HT-29 human colon cells were assessed by MTT and [3H]thymidine incorporation assays. Interleukin-8 (IL-8) expression was stimulated with tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), or lipopolysacharide (LPS). IL-8 was assessed by RT-PCR. Results: BioflorⰒ did not affect viability and proliferation of HT-29 cell. Pretreatment of HT-29 cells with BioflorⰒ blocked IL-8 up-regulation by TNF-α, IL-1β, or LPS to these proinflammatory factors. Conclusions: BioflorⰒ reduces responses to proinflammatory cytokines in human colon cells. (Intestinal Research 2004;2:96-101)
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