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- Original Article Cyclooxgenase-2 Expression in Chronic Inflammatory Bowel Disease: Comparing of Immunohistochemical Stain to Real-time PCR
- Young Sook Park, Nam In Kim, Jun Seok Lee, Jeoung Ho Choi, Chae Young Im, Dae Won Jun, Yun Ju Jo, Seung Hwan Kim, Byung Kwan Son, Won Mee Lee, Eun Kyoung Kim
- Intestinal Research 2008;6(2):128-134. Published online: December 30, 2008
Departments of Internal Medicine and Pathology, Eulji University School of Medicine, Eulji Medical Center, Seoul, Korea
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Abstract
Background/Aims
This study compared the results of cyclooxygenase-2 (COX-2) expression in inflammatory bowel disease and tuberculous colitis as evident by immunochemical staining and real time polymerase chain reaction (PCR). Methods: Patients with ulcerative colitis (n=18), Crohn's disease (n=7), tuberculous colitis (n=7) and 10 normal controls were included. Biopsied colonic mucosa was simultaneously used for immunohistochemical staining and real time PCR. Results: Patients with inflammatory bowel disease and tuberculous colitis showed high COX-2 expression by both methods compared to the normal controls. In Crohn's disease patients, the real time PCR value correlated well staining grade; this correlation was not evident in ulcerative colitis patients. In real time PCR, grossly normal colonic mucosa in ulcerative colitis also showed higher expression of COX-2 than normal mucosa. Conclusions: Real time PCR value of COX-2 is more representative of inflammation state in inflammatory bowel disease than the value from immunohistochemical staining. (Intest Res 2008;6:128-134)
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