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Intestinal Research 2008;6(2):128-134.
Published online December 30, 2008.
Cyclooxgenase-2 Expression in Chronic Inflammatory Bowel Disease: Comparing of Immunohistochemical Stain to Real-time PCR
Young Sook Park, Nam In Kim, Jun Seok Lee, Jeoung Ho Choi, Chae Young Im, Dae Won Jun, Yun Ju Jo, Seung Hwan Kim, Byung Kwan Son, Won Mee Lee, Eun Kyoung Kim
Departments of Internal Medicine and Pathology, Eulji University School of Medicine, Eulji Medical Center, Seoul, Korea
만성 염증성 장질환에서 Cyclooxygenase-2의 발현: 면역조직화학염색과 실시간 중합효소 연쇄반응의 비교
박영숙, 김남인, 이준석, 최정호, 임채영, 전대원, 조윤주, 김성환, 손병관, 이원미, 김은경
을지대학교 의과대학 을지병원 내과학교실, 병리학교실
Abstract
Background/Aims
This study compared the results of cyclooxygenase-2 (COX-2) expression in inflammatory bowel disease and tuberculous colitis as evident by immunochemical staining and real time polymerase chain reaction (PCR). Methods: Patients with ulcerative colitis (n=18), Crohn's disease (n=7), tuberculous colitis (n=7) and 10 normal controls were included. Biopsied colonic mucosa was simultaneously used for immunohistochemical staining and real time PCR. Results: Patients with inflammatory bowel disease and tuberculous colitis showed high COX-2 expression by both methods compared to the normal controls. In Crohn's disease patients, the real time PCR value correlated well staining grade; this correlation was not evident in ulcerative colitis patients. In real time PCR, grossly normal colonic mucosa in ulcerative colitis also showed higher expression of COX-2 than normal mucosa. Conclusions: Real time PCR value of COX-2 is more representative of inflammation state in inflammatory bowel disease than the value from immunohistochemical staining. (Intest Res 2008;6:128-134)
Key Words: Cyclooxygenase 2, Inflammatory Bowel Diseases, Immunohistochemistry, Polymerase Chain Reaction
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