| The Effect of Autonomic Nerve System on Neurotensin Induced Motility in Isolated, Vascularly Perfused Rat Colon |
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Jeong Hoon Ji, Sung Moo Kim, Euikeun Seo, Young Shim Cho, Suk-Hee Yoo, Joung-Ho Han, Hee Bok Chae, Seon Mee Park, Sei Jin Youn |
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Department of Internal Medicine, Division of Gastroenterology and Hepatology, Chungbuk National University College of Medicine, Cheongju, Korea
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| 흰쥐 결장운동에서 Neurotensin의 수축작용과 자율신경계의 영향 |
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지정훈, 김성무, 서의근, 조영심, 유숙희, 한정호, 채희복, 박선미, 윤세진 |
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충북대학교 의과대학 내과학교실 소화기내과 |
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| Abstract |
Background/Aims
Although neurotensin (NT) stimulates colon motility and the passage of intestinal contents, the associated mechanism of action remains unclear. The objective of this study was to investigate the effects of NT on colon motility using isolated rat colon. Methods: Intraluminal pressure was measured at both the proximal and distal portions of the isolated colon. An isolated rat colon was perfused with Krebs solution via the superior mesenteric artery. After stabilization, NT was administered in concentrations of 14, 28, 138 and 276 pM. After pretreatment with phentolamine, propranolol, hexamethonium, atropine or tetrodotoxin, NT was administered at a concentration of 276 pM, and then the intraluminal pressure was monitored. Results: NT significantly increased colon motility at concentrations of 14, 28, 138, and 276 in the proximal colon (25.1±6.5%, 175.4±117.0%, 240.8±115.1% and 252.3±110.6%, respectively) and in the distal colon (35.6±11.8%, 97.5±35.1%, 132.7±36.7% and 212.1±75.2%, respectively). The stimulant effect of NT was more potent in the proximal colon, in a concentration-dependent manner (P＜0.05). The stimulant effect of NT was significantly inhibited by atropine at both the proximal and distal colon and by tetrodotoxin at the proximal colon, but not by tetrodotoxin at the distal colon and not by propranolol, phentolamine, or hexamethonium at both the proximal and distal colon. Conclusions: NT increased colon motility at both the proximal and distal portions of the rat colon. The effects were more prominent at the proximal portion. The results of this study suggest that the stimulant action of NT may be mediated by local cholinergic muscarinic receptors. (Intest Res 2010;8:162-171) |
| Key Words:
Neurotensin (NT), Colon Motility, Cholinergic |
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